Implementation of a continuous scanning procedure and a line scan camera for thin-sheet laser imaging microscopy

Peter Schacht, Shane B. Johnson, Peter A. Santi

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We report development of a continuous scanning procedure and the use of a time delay integration (TDI) line scan camera for a light-sheet based microscope called a thin-sheet laser imaging microscope (TSLIM). TSLIM is an optimized version of a light-sheet fluorescent microscope that previously used a start/stop scanning procedure to move the specimen through the thinnest portion of a light-sheet and stitched the image columns together to produce a well-focused composite image. In this paper, hardware and software enhancements to TSLIM are described that allow for dual sided, dual illumination lasers, and continuous scanning of the specimen using either a full-frame CCD camera and a TDI line scan camera. These enhancements provided a ~70% reduction in the time required for composite image generation and a ~63% reduction in photobleaching of the specimen compared to the start/stop procedure.

Original languageEnglish (US)
Pages (from-to)598-609
Number of pages12
JournalBiomedical Optics Express
Volume1
Issue number2
DOIs
StatePublished - Sep 1 2010

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