Implantation of completely biological engineered grafts following decellularization into the sheep femoral artery

Zeeshan H. Syedain, Lee A. Meier, Mathew T. Lahti, Sandra L. Johnson, Robert T. Tranquillo

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential of these unique arterial allografts. The 4-mm-diameter, 2-3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000?mmHg and the same compliance as the ovine femoral artery. Grafts were implanted interpositionally in the femoral artery of six sheep (n=9), with contralateral sham controls (n=3). At 8 weeks (n=5) and 24 weeks (n=4), all grafts were patent and showed no evidence of dilatation or mineralization. Mid-graft lumen diameter was unchanged. Extensive recellularization occurred, with most cells expressing αSMA. Endothelialization was complete by 24 weeks with elastin deposition evident. These completely biological grafts possessed circumferential alignment/mechanical anisotropy characteristic of native arteries and were cultured only 5 weeks prior to decellularization and storage as "off-the-shelf" grafts.

Original languageEnglish (US)
Pages (from-to)1726-1734
Number of pages9
JournalTissue Engineering - Part A
Volume20
Issue number11-12
DOIs
StatePublished - Jun 1 2014

Fingerprint

Femoral Artery
Grafts
Sheep
Transplants
Allografts
Elastin
Anisotropy
Fibroblasts
Fibrin
Compliance
Dilatation
Gels
Arteries
Cells
Tissue
Pressure
Skin

Cite this

Implantation of completely biological engineered grafts following decellularization into the sheep femoral artery. / Syedain, Zeeshan H.; Meier, Lee A.; Lahti, Mathew T.; Johnson, Sandra L.; Tranquillo, Robert T.

In: Tissue Engineering - Part A, Vol. 20, No. 11-12, 01.06.2014, p. 1726-1734.

Research output: Contribution to journalArticle

Syedain, Zeeshan H. ; Meier, Lee A. ; Lahti, Mathew T. ; Johnson, Sandra L. ; Tranquillo, Robert T. / Implantation of completely biological engineered grafts following decellularization into the sheep femoral artery. In: Tissue Engineering - Part A. 2014 ; Vol. 20, No. 11-12. pp. 1726-1734.
@article{8d15bd8493f8496d841dd6c8615bfae0,
title = "Implantation of completely biological engineered grafts following decellularization into the sheep femoral artery",
abstract = "The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential of these unique arterial allografts. The 4-mm-diameter, 2-3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000?mmHg and the same compliance as the ovine femoral artery. Grafts were implanted interpositionally in the femoral artery of six sheep (n=9), with contralateral sham controls (n=3). At 8 weeks (n=5) and 24 weeks (n=4), all grafts were patent and showed no evidence of dilatation or mineralization. Mid-graft lumen diameter was unchanged. Extensive recellularization occurred, with most cells expressing αSMA. Endothelialization was complete by 24 weeks with elastin deposition evident. These completely biological grafts possessed circumferential alignment/mechanical anisotropy characteristic of native arteries and were cultured only 5 weeks prior to decellularization and storage as {"}off-the-shelf{"} grafts.",
author = "Syedain, {Zeeshan H.} and Meier, {Lee A.} and Lahti, {Mathew T.} and Johnson, {Sandra L.} and Tranquillo, {Robert T.}",
year = "2014",
month = "6",
day = "1",
doi = "10.1089/ten.tea.2013.0550",
language = "English (US)",
volume = "20",
pages = "1726--1734",
journal = "Tissue Engineering - Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "11-12",

}

TY - JOUR

T1 - Implantation of completely biological engineered grafts following decellularization into the sheep femoral artery

AU - Syedain, Zeeshan H.

AU - Meier, Lee A.

AU - Lahti, Mathew T.

AU - Johnson, Sandra L.

AU - Tranquillo, Robert T.

PY - 2014/6/1

Y1 - 2014/6/1

N2 - The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential of these unique arterial allografts. The 4-mm-diameter, 2-3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000?mmHg and the same compliance as the ovine femoral artery. Grafts were implanted interpositionally in the femoral artery of six sheep (n=9), with contralateral sham controls (n=3). At 8 weeks (n=5) and 24 weeks (n=4), all grafts were patent and showed no evidence of dilatation or mineralization. Mid-graft lumen diameter was unchanged. Extensive recellularization occurred, with most cells expressing αSMA. Endothelialization was complete by 24 weeks with elastin deposition evident. These completely biological grafts possessed circumferential alignment/mechanical anisotropy characteristic of native arteries and were cultured only 5 weeks prior to decellularization and storage as "off-the-shelf" grafts.

AB - The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential of these unique arterial allografts. The 4-mm-diameter, 2-3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000?mmHg and the same compliance as the ovine femoral artery. Grafts were implanted interpositionally in the femoral artery of six sheep (n=9), with contralateral sham controls (n=3). At 8 weeks (n=5) and 24 weeks (n=4), all grafts were patent and showed no evidence of dilatation or mineralization. Mid-graft lumen diameter was unchanged. Extensive recellularization occurred, with most cells expressing αSMA. Endothelialization was complete by 24 weeks with elastin deposition evident. These completely biological grafts possessed circumferential alignment/mechanical anisotropy characteristic of native arteries and were cultured only 5 weeks prior to decellularization and storage as "off-the-shelf" grafts.

UR - http://www.scopus.com/inward/record.url?scp=84901217965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901217965&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2013.0550

DO - 10.1089/ten.tea.2013.0550

M3 - Article

VL - 20

SP - 1726

EP - 1734

JO - Tissue Engineering - Part A

JF - Tissue Engineering - Part A

SN - 1937-3341

IS - 11-12

ER -