Implantable microelectrode arrays for simultaneous electrophysiological and neurochemical recordings

Matthew D. Johnson, Robert K. Franklin, Matthew D. Gibson, Richard B. Brown, Daryl R. Kipke

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

Implantable microfabricated microelectrode arrays represent a versatile and powerful tool to record electrophysiological activity across multiple spatial locations in the brain. Spikes and field potentials, however, correspond to only a fraction of the physiological information available at the neural interface. In urethane-anesthetized rats, microfabricated microelectrode arrays were implanted acutely for simultaneous recording of striatal local field potentials, spikes, and electrically evoked dopamine overflow on the same spatiotemporal scale. During these multi-modal recordings we observed (1) that the amperometric method used to detect dopamine did not significantly influence electrophysiological activity, (2) that electrical stimulation in the medial forebrain bundle (MFB) region resulted in electrochemically transduced dopamine transients in the striatum that were spatially heterogeneous within at least 200 μm, and (3) following MFB stimulation, dopamine levels and electrophysiological activity within the striatum exhibited similar temporal profiles. These neural probes are capable of incorporating customized microelectrode geometries and configurations, which may be useful for examining specific spatiotemporal relationships between electrical and chemical signaling in the brain.

Original languageEnglish (US)
Pages (from-to)62-70
Number of pages9
JournalJournal of Neuroscience Methods
Volume174
Issue number1
DOIs
StatePublished - Sep 15 2008

Bibliographical note

Funding Information:
This research was supported by a National Institutes of Health NIBIB grant (R21-EB005022) and the NSF Center for Wireless Integrated Microsystems (EEC-9986866). We thank Kat Scott and Charles Wood for technical support; and Jamille Hetke, Ning Gulari, and Brendan Casey for assistance with microfabrication and probe assembly.

Keywords

  • Amperometry
  • Dopamine
  • Local field potentials
  • Neural probe
  • Spike recordings
  • Striatum

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