Impaired resolution of inflammation in the Endoglin heterozygous mouse model of chronic colitis

Madonna R. Peter, Mirjana Jerkic, Valentin Sotov, David N. Douda, Daniela S. Ardelean, Niousha Ghamami, Flavia Lakschevitz, Meraj A. Khan, Susan J. Robertson, Michael Glogauer, Dana J. Philpott, Nades Palaniyar, Michelle Letarte

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20 Scopus citations

Abstract

Endoglin is a coreceptor of the TGF-β superfamily predominantly expressed on the vascular endothelium and selective subsets of immune cells. We previously demonstrated that Endoglin heterozygous (Eng +/-) mice subjected to dextran sulfate sodium (DSS) developed persistent gut inflammation and pathological angiogenesis. We now report that colitic Eng +/- mice have low colonic levels of active TGF-β1, which was associated with reduced expression of thrombospondin-1, an angiostatic factor known to activate TGF-β1. We also demonstrate dysregulated expression of BMPER and follistatin, which are extracellular regulators of the TGF-β superfamily that modulate angiogenesis and inflammation. Heightened colonic levels of the neutrophil chemoattractant and proangiogenic factor, CXCL1, were also observed in DSS-treated Eng +/- mice. Interestingly, despite increased macrophage and neutrophil infiltration, a gut-specific reduction in expression of the key phagocytic respiratory burst enzymes, NADPH oxidase 2 (Nox-2) and myeloperoxidase, was seen in Eng +/- mice undergoing persistent inflammation. Taken together, these findings suggest that endoglin is required for TGF-β superfamily mediated resolution of inflammation and fully functional myeloid cells.

Original languageEnglish (US)
Article number767185
JournalMediators of inflammation
Volume2014
DOIs
StatePublished - 2014
Externally publishedYes

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