Impaired function of the tonoplast-localized sucrose transporter in rice, OsSUT2, limits the transport of vacuolar reserve sucrose and affects plant growth

Joon Seob Eom, Jung Il Cho, Anke Reinders, Sang Won Lee, Youngchul Yoo, Pham Quoc Tuan, Sang Bong Choi, Geul Bang, Youn Il Park, Man Ho Cho, Seong Hee Bhoo, Gynheung An, Tae Ryong Hahn, John M Ward, Jong Seong Jeon

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Abstract

Physiological functions of sucrose (Suc) transporters (SUTs) localized to the tonoplast in higher plants are poorly understood. We here report the isolation and characterization of a mutation in the rice (Oryza sativa) OsSUT2 gene. Expression of OsSUT2- green fluorescent protein in rice revealed that OsSUT2 localizes to the tonoplast. Analysis of the OsSUT2 promoter::bglucuronidase transgenic rice indicated that this gene is highly expressed in leaf mesophyll cells, emerging lateral roots, pedicels of fertilized spikelets, and cross cell layers of seed coats. Results of Suc transport assays in yeast were consistent with a H+-Suc symport mechanism, suggesting that OsSUT2 functions in Suc uptake from the vacuole. The ossut2 mutant exhibited a growth retardation phenotype with a significant reduction in tiller number, plant height, 1,000-grain weight, and root dry weight compared with the controls, the wild type, and complemented transgenic lines. Analysis of primary carbon metabolites revealed that ossut2 accumulated more Suc, glucose, and fructose in the leaves than the controls. Further sugar export analysis of detached leaves indicated that ossut2 had a significantly decreased sugar export ability compared with the controls. These results suggest that OsSUT2 is involved in Suc transport across the tonoplast from the vacuole lumen to the cytosol in rice, playing an essential role in sugar export from the source leaves to sink organs.

Original languageEnglish (US)
Pages (from-to)109-119
Number of pages11
JournalPlant physiology
Volume157
Issue number1
DOIs
StatePublished - Sep 2011

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tonoplast
Sucrose
transporters
plant growth
sucrose
rice
Growth
Vacuoles
sugars
vacuoles
leaves
genetically modified organisms
Mesophyll Cells
Weights and Measures
pedicel
Ion Transport
Green Fluorescent Proteins
Fructose
green fluorescent protein
cytosol

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Impaired function of the tonoplast-localized sucrose transporter in rice, OsSUT2, limits the transport of vacuolar reserve sucrose and affects plant growth. / Eom, Joon Seob; Cho, Jung Il; Reinders, Anke; Lee, Sang Won; Yoo, Youngchul; Tuan, Pham Quoc; Choi, Sang Bong; Bang, Geul; Park, Youn Il; Cho, Man Ho; Bhoo, Seong Hee; An, Gynheung; Hahn, Tae Ryong; Ward, John M; Jeon, Jong Seong.

In: Plant physiology, Vol. 157, No. 1, 09.2011, p. 109-119.

Research output: Contribution to journalArticle

Eom, JS, Cho, JI, Reinders, A, Lee, SW, Yoo, Y, Tuan, PQ, Choi, SB, Bang, G, Park, YI, Cho, MH, Bhoo, SH, An, G, Hahn, TR, Ward, JM & Jeon, JS 2011, 'Impaired function of the tonoplast-localized sucrose transporter in rice, OsSUT2, limits the transport of vacuolar reserve sucrose and affects plant growth', Plant physiology, vol. 157, no. 1, pp. 109-119. https://doi.org/10.1104/pp.111.176982
Eom, Joon Seob ; Cho, Jung Il ; Reinders, Anke ; Lee, Sang Won ; Yoo, Youngchul ; Tuan, Pham Quoc ; Choi, Sang Bong ; Bang, Geul ; Park, Youn Il ; Cho, Man Ho ; Bhoo, Seong Hee ; An, Gynheung ; Hahn, Tae Ryong ; Ward, John M ; Jeon, Jong Seong. / Impaired function of the tonoplast-localized sucrose transporter in rice, OsSUT2, limits the transport of vacuolar reserve sucrose and affects plant growth. In: Plant physiology. 2011 ; Vol. 157, No. 1. pp. 109-119.
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AU - Lee, Sang Won

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AU - Tuan, Pham Quoc

AU - Choi, Sang Bong

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AU - Cho, Man Ho

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