Abstract
While grazing exclusion is thought to drive soil nutrient transport and cycling, and reduce soil compaction, its direct impact on microbial community composition remains unclear. In this study, we examined the impact of grazing exclusion on abundance and composition of soil microbial (bacterial, archaeal, and fungal) communities, especially those associated with nutrient cycling. We surveyed soil physicochemical properties and litter mass, at sites undergoing varying durations of grazing exclusion (0–34 years) in a semiarid grassland. Using next-generation amplicon sequencing, we further characterized variations in the composition and diversity of soil microbial communities associated with grazing exclusion and soil depths, as well as subsequent changes in physicochemical properties. Most soil physicochemical parameter values significantly increased as the result of long-term grazing exclusion, and these properties were associated with variation in composition and diversity of microbial communities. Notably, the relative abundances of microbial families associated with C cycling (e.g., Chitinophagaceae) increased with an increase in nutrient availability following grazing exclusion. The abundance of the archaeal ammonia-oxidizing Nitrososphaerae increased with decreasing concentration of ammonium among samples. Likewise, fungal communities were also associated with the shifts in nutrient concentrations, although the majority of fungi could not be classified to the species level. Nitrate concentration also played a critical role in shaping bacterial, archaeal, or fungal communities. Moreover, bacterial and archaeal communities had a greater mean Shannon index in 0–10-cm than those in 10–20-cm soil layer. Results of this study provide novel insights regarding how the length of grazing exclusion and soil depth influence nutrient gradients and microbial community composition associated with nutrient cycling.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 121-134 |
| Number of pages | 14 |
| Journal | Biology and Fertility of Soils |
| Volume | 55 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2019 |
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Keywords
- Bacteria
- Exclusion-grazing
- Fungi
- Next-generation amplicon sequencing
- Soil nutrients
Cite this
Impact of long-term grazing exclusion on soil microbial community composition and nutrient availability. / Wang, Zhe; Zhang, Qian; Staley, Christopher M; Gao, Hailong; Ishii, Satoshi; Wei, Xiaorong; Liu, Jian; Cheng, Jimin; Hao, Mingde; Sadowsky, Michael J.
In: Biology and Fertility of Soils, Vol. 55, No. 2, 01.02.2019, p. 121-134.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Impact of long-term grazing exclusion on soil microbial community composition and nutrient availability
AU - Wang, Zhe
AU - Zhang, Qian
AU - Staley, Christopher M
AU - Gao, Hailong
AU - Ishii, Satoshi
AU - Wei, Xiaorong
AU - Liu, Jian
AU - Cheng, Jimin
AU - Hao, Mingde
AU - Sadowsky, Michael J
PY - 2019/2/1
Y1 - 2019/2/1
N2 - While grazing exclusion is thought to drive soil nutrient transport and cycling, and reduce soil compaction, its direct impact on microbial community composition remains unclear. In this study, we examined the impact of grazing exclusion on abundance and composition of soil microbial (bacterial, archaeal, and fungal) communities, especially those associated with nutrient cycling. We surveyed soil physicochemical properties and litter mass, at sites undergoing varying durations of grazing exclusion (0–34 years) in a semiarid grassland. Using next-generation amplicon sequencing, we further characterized variations in the composition and diversity of soil microbial communities associated with grazing exclusion and soil depths, as well as subsequent changes in physicochemical properties. Most soil physicochemical parameter values significantly increased as the result of long-term grazing exclusion, and these properties were associated with variation in composition and diversity of microbial communities. Notably, the relative abundances of microbial families associated with C cycling (e.g., Chitinophagaceae) increased with an increase in nutrient availability following grazing exclusion. The abundance of the archaeal ammonia-oxidizing Nitrososphaerae increased with decreasing concentration of ammonium among samples. Likewise, fungal communities were also associated with the shifts in nutrient concentrations, although the majority of fungi could not be classified to the species level. Nitrate concentration also played a critical role in shaping bacterial, archaeal, or fungal communities. Moreover, bacterial and archaeal communities had a greater mean Shannon index in 0–10-cm than those in 10–20-cm soil layer. Results of this study provide novel insights regarding how the length of grazing exclusion and soil depth influence nutrient gradients and microbial community composition associated with nutrient cycling.
AB - While grazing exclusion is thought to drive soil nutrient transport and cycling, and reduce soil compaction, its direct impact on microbial community composition remains unclear. In this study, we examined the impact of grazing exclusion on abundance and composition of soil microbial (bacterial, archaeal, and fungal) communities, especially those associated with nutrient cycling. We surveyed soil physicochemical properties and litter mass, at sites undergoing varying durations of grazing exclusion (0–34 years) in a semiarid grassland. Using next-generation amplicon sequencing, we further characterized variations in the composition and diversity of soil microbial communities associated with grazing exclusion and soil depths, as well as subsequent changes in physicochemical properties. Most soil physicochemical parameter values significantly increased as the result of long-term grazing exclusion, and these properties were associated with variation in composition and diversity of microbial communities. Notably, the relative abundances of microbial families associated with C cycling (e.g., Chitinophagaceae) increased with an increase in nutrient availability following grazing exclusion. The abundance of the archaeal ammonia-oxidizing Nitrososphaerae increased with decreasing concentration of ammonium among samples. Likewise, fungal communities were also associated with the shifts in nutrient concentrations, although the majority of fungi could not be classified to the species level. Nitrate concentration also played a critical role in shaping bacterial, archaeal, or fungal communities. Moreover, bacterial and archaeal communities had a greater mean Shannon index in 0–10-cm than those in 10–20-cm soil layer. Results of this study provide novel insights regarding how the length of grazing exclusion and soil depth influence nutrient gradients and microbial community composition associated with nutrient cycling.
KW - Bacteria
KW - Exclusion-grazing
KW - Fungi
KW - Next-generation amplicon sequencing
KW - Soil nutrients
UR - http://www.scopus.com/inward/record.url?scp=85059626447&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059626447&partnerID=8YFLogxK
U2 - 10.1007/s00374-018-01336-5
DO - 10.1007/s00374-018-01336-5
M3 - Article
AN - SCOPUS:85059626447
VL - 55
SP - 121
EP - 134
JO - Biology and Fertility of Soils
JF - Biology and Fertility of Soils
SN - 0178-2762
IS - 2
ER -