Impact of long-term cryopreservation on single umbilical cord blood transplantation outcomes

Richard Mitchell, John E. Wagner, Claudio G. Brunstein, Qing Cao, David H. McKenna, Troy C. Lund, Michael R. Verneris

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Umbilical cord blood (UCB) may be collected and cryopreserved for years before use. Invitro and murine models suggest that the duration of storage does not affect UCB progenitor cell performance; however, the impact of UCB age on clinical outcomes has not been definitely defined. This study sought to determine the effect of UCB unit cryopreservation time on hematopoietic potency. We analyzed 288 single UCB units used for transplantation from 1992 to 2013, with unit cryopreservation time ranging from .08 to 11.07 years. UCB unit post-thaw characteristics were examined, including percent recovery of total nucleated cells (TNC). The number of years the UCB unit spent in cryopreservation had no impact on TNC recovery nor UCB unit post-thaw viability. Duration of cryopreservation also had no impact on neutrophil or platelet engraftment in single UCB transplantations. These results show that UCB units can undergo cryopreservation for at least 10 years with no impact on clinical outcomes.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalBiology of Blood and Marrow Transplantation
Issue number1
StatePublished - Jan 1 2015

Bibliographical note

Publisher Copyright:
© 2015 American Society for Blood and Marrow Transplantation.


  • Neutrophil recovery
  • UCB storage time
  • UCB transplantation
  • Umbilical cord blood (UCB)


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