Impact of inhibiting activity of indole inhibitors on phospholipid hydrolysis by phospholipase A2

Xiuhong Zhai, Gerald Brezesinski, Helmuth Möhwald, Junbai Li

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The influence of two indole inhibitors on the hydrolysis reaction of l-dipalmitoylphosphatidylcholine (l-DPPC) catalyzed by phospholipase A 2 (PLA2) has been studied in the coexistence region using surface pressure as function of time (π-t) and Brewster angle microscopy (BAM) measurements. Similar experiments have been performed with l-DPPC vesicles made by ultrasonication method using transmission electron microscopy (TEM). Comparing the π-t curves and BAM images, which show defected domains after a certain reaction time, as well as TEM images of defected vesicles, one can conclude that the two indole inhibitors have different abilities in reducing the activity of PLA2. The described experiments show that BAM and TEM can be successfully used to study the influence of newly designed inhibitors on the hydrolysis reaction catalyzed by PLA2.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume256
Issue number1 SPEC. ISS.
DOIs
StatePublished - Apr 1 2005

Bibliographical note

Funding Information:
We acknowledge the financial support of the National Nature Science Foundation of China (NNSFC 29925307), the Major State Basic Research Development Program (973, Grant No. G2000078103), the Chinese Academy of Sciences as well as the collaboration project of the German Max Planck Society.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

  • Catalyzed
  • Indole
  • PLA

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