Impact of full range of amylose contents on the architecture of starch granules*

Avi Goldstein, George Annor, Jean Luc Putaux, Kim H. Hebelstrup, Andreas Blennow, Eric Bertoft

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The effects of amylose deposition on crystalline regions of barley starch granules were studied in granules containing zero to 99.1% amylose using “waxy” (WBS, 0% amylose), normal (NBS, 18% amylose) and amylose-only barley lines (AOS, 99.1% amylose). The effects were probed after hydrolysis of amorphous regions of starch granules in dilute HCl generating lintners, which typically represent the crystalline lamella of starch granules. Compared to NBS and WBS, AOS granules exhibited an irregular, multilobular morphology with a rough surface texture. AOS displayed lower rates of acid hydrolysis than WBS, and AOS reached a plateau at ∼45 wt% acid hydrolysis. High-performance anion-exchange chromatography of lintners at equivalent levels of hydrolysis (45 wt%) revealed the average degree of polymerization (DP) of AOS lintners was 21, substantially smaller than that of NBS and WBS (DP 42). AOS lintners contained the lowest number of chains (NC) per molecule (1.1) compared to NBS (2.8) and WBS (3.3) and the average chain length of AOS, NBS and WBS lintners was 19, 15 and 13, respectively. Hence, both NC and the average chain length correlated with amylose content. The size distribution profile of AOS lintners revealed a repeat motif in the molecules corresponding to 5–6 glucose residues.

Original languageEnglish (US)
Pages (from-to)305-318
Number of pages14
JournalInternational Journal of Biological Macromolecules
Volume89
DOIs
StatePublished - Aug 1 2016

Keywords

  • Acid hydrolysis
  • Amylose
  • Barley
  • Crystalline polysaccharides
  • Lintnerization
  • Starch

PubMed: MeSH publication types

  • Journal Article

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