Immunoregulatory characteristics of the in vitro anti-ssDNA response

Colin C. Anderson, Angela Panoskaltsis, Nicholas R StC Sinclair

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Continuous blockade of B-cell antigen receptors (BCRs) with Fab αsIg prevents the anti-ssDNA response of high, but not low, density B cells. Signaling via the BCRs, by prior exposure to crosslinking F(ab′)2 αsIg, had no effect on the spontaneous anti-DNA response, but prevented a lipopolysaccharide-induced anti-DNA response. Pretreatment with intact αsIg, which provides exogenously derived Fc signals, reduced the response. An Fc-signal-blocking agent, F(ab′)2 anti-IgG-Fc antibody, increased the number of anti-DNA antibody-forming cells produced in the absence of exogenous IgG anti-ssDNA antibody. Thus, activation is dependent on the availability of the BCRs, prior BCR crosslinking does not interfere with activation, and endogenous IgG anti-ssDNA antibody limits the activation of anti-ssDNA-specific B cells most of which are T-cell independent. These results indicate that the anti-ssDNA response is driven through the BCR.

Original languageEnglish (US)
Pages (from-to)349-357
Number of pages9
JournalImmunologic Research
Volume12
Issue number4
DOIs
StatePublished - Dec 1 1993

Keywords

  • Anti-ssDNA antibody
  • Antigen receptor, B-cell
  • B-cell Fc receptor
  • End-product feedback
  • Fc signaling
  • Fc-signal blockade
  • Lipopolysaccharide activation

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