Abstract
It is well known that immunoproteasome generates peptides for MHC Class I occupancy and recognition by cytotoxic T lymphocytes (CTL). The present study focused on evidence for alternative roles for immunoproteasome. Retina and brain were analyzed for expression of immunoproteasome subunits using immunohistochemistry and western blotting under normal conditions and after injury/stress induced by CTL attack on glia (brain) or neurons (retina). Normal retina expressed substantial levels of immunoproteasome in glia, neurons, and retinal pigment epithelium. The basal level of immunoproteasome in retina was two-fold higher than in brain; CTL-induced retinal injury further up-regulated immunoproteasome expression. Immunoproteasome up-regulation was also observed in injured brain and corresponded with expression in Purkinje cells, microglia, astrocytes, and oligodendrocytes. These results suggest that the normal environment of the retina is sufficiently challenging to require on-going expression of immunoproteasome. Further, immunoproteasome up-regulation with retinal and brain injury implies a role in neuronal protection and/or repair of damage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 158-169 |
| Number of pages | 12 |
| Journal | Journal of Neurochemistry |
| Volume | 106 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 2008 |
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Keywords
- Brain
- Immunoproteasome
- Neuronal injury
- Proteasome
- Retina
Cite this
Immunoproteasome responds to injury in the retina and brain. / Ferrington, Deborah A; Hussong, Stacy A.; Roehrich, Heidi; Kapphahn, Becky; Kavanaugh, Shannon M.; Heuss, Neal D; Gregerson, Dale S.
In: Journal of Neurochemistry, Vol. 106, No. 1, 01.07.2008, p. 158-169.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunoproteasome responds to injury in the retina and brain
AU - Ferrington, Deborah A
AU - Hussong, Stacy A.
AU - Roehrich, Heidi
AU - Kapphahn, Becky
AU - Kavanaugh, Shannon M.
AU - Heuss, Neal D
AU - Gregerson, Dale S
PY - 2008/7/1
Y1 - 2008/7/1
N2 - It is well known that immunoproteasome generates peptides for MHC Class I occupancy and recognition by cytotoxic T lymphocytes (CTL). The present study focused on evidence for alternative roles for immunoproteasome. Retina and brain were analyzed for expression of immunoproteasome subunits using immunohistochemistry and western blotting under normal conditions and after injury/stress induced by CTL attack on glia (brain) or neurons (retina). Normal retina expressed substantial levels of immunoproteasome in glia, neurons, and retinal pigment epithelium. The basal level of immunoproteasome in retina was two-fold higher than in brain; CTL-induced retinal injury further up-regulated immunoproteasome expression. Immunoproteasome up-regulation was also observed in injured brain and corresponded with expression in Purkinje cells, microglia, astrocytes, and oligodendrocytes. These results suggest that the normal environment of the retina is sufficiently challenging to require on-going expression of immunoproteasome. Further, immunoproteasome up-regulation with retinal and brain injury implies a role in neuronal protection and/or repair of damage.
AB - It is well known that immunoproteasome generates peptides for MHC Class I occupancy and recognition by cytotoxic T lymphocytes (CTL). The present study focused on evidence for alternative roles for immunoproteasome. Retina and brain were analyzed for expression of immunoproteasome subunits using immunohistochemistry and western blotting under normal conditions and after injury/stress induced by CTL attack on glia (brain) or neurons (retina). Normal retina expressed substantial levels of immunoproteasome in glia, neurons, and retinal pigment epithelium. The basal level of immunoproteasome in retina was two-fold higher than in brain; CTL-induced retinal injury further up-regulated immunoproteasome expression. Immunoproteasome up-regulation was also observed in injured brain and corresponded with expression in Purkinje cells, microglia, astrocytes, and oligodendrocytes. These results suggest that the normal environment of the retina is sufficiently challenging to require on-going expression of immunoproteasome. Further, immunoproteasome up-regulation with retinal and brain injury implies a role in neuronal protection and/or repair of damage.
KW - Brain
KW - Immunoproteasome
KW - Neuronal injury
KW - Proteasome
KW - Retina
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UR - http://www.scopus.com/inward/citedby.url?scp=45249092950&partnerID=8YFLogxK
U2 - 10.1111/j.1471-4159.2008.05345.x
DO - 10.1111/j.1471-4159.2008.05345.x
M3 - Article
C2 - 18346202
AN - SCOPUS:45249092950
VL - 106
SP - 158
EP - 169
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 1
ER -