Immunophysiological studies of interleukin-2 and canine lymphocytes

Stuart C. Helfand, Jaime F. Modiano, Peter C. Nowell

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27 Citations (Scopus)

Abstract

Interleukin-2-dependent pathways of lymphocyte activation were investigated in canine peripheral blood lymphocytes (PBL) following stimulation with T-cell mitogens including phytohemagglutinin, phorbol ester (TPA), calcium ionophore (ionomycin), and human recombinant interleukin-2 (hrIL-2). The ability of the stimulated cells to produce interleukin-2 (IL-2) was determined using murine indicator cell lines. IL-2 receptor expression by mitogen-stimulated canine PBL was confirmed by the binding of hrlL-2 with high affinity, and with characteristics comparable to those of the human and murine IL-2 receptor. Examination of serum and PBL from two dogs that were treated with hrlL-2 and human recombinant tumor necrosis factor for systemic mast cell tumors showed that in one dog, IL-2 could be measured in the serum. Concurrently, the in vitro mitogenic response of this dog's PBL to hrlL-2 occurred earlier, possibly reflecting an increase in the relative number of IL-2-responsive cells within the PBL population.

Original languageEnglish (US)
Pages (from-to)1-16
Number of pages16
JournalVeterinary Immunology and Immunopathology
Volume33
Issue number1-2
DOIs
StatePublished - Jan 1 1992

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interleukin-2
Interleukin-2
Canidae
lymphocytes
Lymphocytes
dogs
Interleukin-2 Receptors
Dogs
Lymphocyte Activation
blood
Mitogens
lymphocyte proliferation
blood serum
Ionomycin
Calcium Ionophores
Phytohemagglutinins
Phorbol Esters
Serum
Mast Cells
receptors

Cite this

Immunophysiological studies of interleukin-2 and canine lymphocytes. / Helfand, Stuart C.; Modiano, Jaime F.; Nowell, Peter C.

In: Veterinary Immunology and Immunopathology, Vol. 33, No. 1-2, 01.01.1992, p. 1-16.

Research output: Contribution to journalArticle

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