Immunoperoxidase staining of Streptococcus mutans

P. Berthold, D. Bratthall, C. H. Berthold

Research output: Contribution to journalArticlepeer-review

Abstract

Cells from two scrologically different strains of Streptococcus mutans strain B13 and strain IB were treated immuno-histochemically for electron microscopy. Both strains were incubated first with specific rabbit anti-B13 serum and then with sheep anti-rabbit serum labelled with horse-radish peroxidase and finally histochemically stained for peroxidase activity. Strain B13 treated with the specific antiserum and exposed to the complete immuno-histochemical procedure revealed a halo of moderate electron-dense material outside the cell wall. All controls were negative.

Original languageEnglish (US)
Pages (from-to)1227-1230,IN29
JournalArchives of Oral Biology
Volume19
Issue number12
DOIs
StatePublished - Dec 1974

Bibliographical note

Funding Information:
Acknowledgmrnts-This research was supported in part by grants from the School of Dentistry, Karolmska Institutet (Reservations-anslaget) and in part by a grant from Patent-medelsfonden fh;r Odontologisk Profylaxforskning. The material and results were presented in part at the 52nd General Session. International Association for Dental Research. Atlanta, Georgia, U.S.A.. 1973.

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