The application of an immunoperoxidase method to labeling of Streptococcus mutans subsp. sobrinus for scanning electron microscopy is described. Bacteria from four separate cultures were fixed in 2.5% buffered glutaraldehyde solution. The specimens were treated in accordance with an indirect immunolabeling procedure using a rabbit anti-S. mutans subsp. sobrinus γglobulin. Labeled bacteria were equipped with a layer of small, distinct globules of horseradish peroxidase reaction product. Bacteria exposed to control incubations were not equipped with this layer.
- Horseradish peroxidase