Immunoperoxidase labeling of streptococcus mutans for scanning electron microscopy

Peter Berthold

Research output: Contribution to journalArticle

Abstract

The application of an immunoperoxidase method to labeling of Streptococcus mutans subsp. sobrinus for scanning electron microscopy is described. Bacteria from four separate cultures were fixed in 2.5% buffered glutaraldehyde solution. The specimens were treated in accordance with an indirect immunolabeling procedure using a rabbit anti-S. mutans subsp. sobrinus γglobulin. Labeled bacteria were equipped with a layer of small, distinct globules of horseradish peroxidase reaction product. Bacteria exposed to control incubations were not equipped with this layer.

Original languageEnglish (US)
Pages (from-to)125-128
Number of pages4
JournalActa Odontologica Scandinavica
Volume41
Issue number2
DOIs
StatePublished - Jan 1 1983

Keywords

  • Bacteria
  • Horseradish peroxidase
  • Immunocytochemistry
  • Ultrastructure

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