Immunological demonstration of a unique 3,4-dihydroxyphenylacetate 2,3- dioxygenase in soil Arthrobacter strains

P. E. Olson, B. Qi, Larry Que, Lawrence P Wackett

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Many bacteria biosynthesize 3,4-dihydroxyphenylacetate 2,3-dioxygenases for growth on aromatic acids, but gram-negative organisms have been most extensively studied. A gram-positive strain containing 2,3-dioxygenase activity was identified as Arthrobacter strain Mn-1. The 2,3-dioxygenase from strain Mn-1 was purified to homogeneity by fast protein liquid chromatography with a Mono Q anion-exchange column. Rabbit polyclonal antidioxygenase antibodies were prepared. Ouchterlony double-diffusion and Western blotting (immunoblotting) protocols were used to probe the distribution of the Mn-1 dioxygenase antigen in soil bacteria. Fourteen 2,3-dioxygenase-containing Bacillus and Pseudomonas strains did not contain immunologically cross- reactive proteins. Six of eight Arthrobacter strains contained 2,3- dioxygenase activity, and all of them produced cross-reactive proteins. The data presented here suggest that a unique type of dioxygenase is geographically widespread but is taxonomically confined to Arthrobacter soil bacteria.

Original languageEnglish (US)
Pages (from-to)2820-2826
Number of pages7
JournalApplied and environmental microbiology
Volume58
Issue number9
DOIs
StatePublished - 1992

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