Immunohistochemical studies of peptidergic neurons in the dorsal horn of the spinal cord

The indirect immunofluorescence technique was used to localize substance P, somatostatin, methionine-enkephalin, neurotensin, and oxytocin in the dorsal horn of the rat spinal cord. The unique distribution of each peptide is described and the relative amount of each peptide in laminae I-III of the dorsal horn and of the dorsal part of the lateral funniculus qualitatively assessed. Colchicine treatment and dorsal rhizotomy were used to determine, in part, the origin of immunoreactive fibers and terminals observed in the dorsal horn.