Immunohistochemical method for the simultaneous demonstration of three proteins in EDTA Decalcified paraffin embedded bone sections

Hermina M. Tulli, Cathy S. Carlson, Manuel J. Jayo, Larry W. Fisher, Kenneth G. Mann

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4 Scopus citations

Abstract

Immunohistochemistry was used to demonstrate the presence of three proteins (actin, osteocalcin and alkaline phosphatase) within the same ethylenediaminetetraacetic acid (EDTA) decalcified and paraffin embedded bone tissue section. EDTA chelated calcium ions, causing endogenous alkaline phosphatase (ALP) to be inactivated. By ablating endogenous ALP activity through chelation, we were able to use an ALP based detection system before reactivation of the endogenous enzyme with magnesium chloride. After reactivation, endogenous ALP itself was demonstrated immunohistochemically. (The J Histotechnol 15:93, 1992).

Original languageEnglish (US)
Pages (from-to)93-97
Number of pages5
JournalJournal of Histotechnology
Volume15
Issue number2
DOIs
StatePublished - Jun 1992

Keywords

  • Actin
  • Alkaline phosphatase
  • Bone
  • EDTA
  • Immunohistochemistry
  • Osteocalcin

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