Immunohistochemical distribution of extracellular matrix components and keratin in experimentally induced otitis media

Teruhiko Harada, Youngki Kim, Steven K. Juhn, Yasuo Sakakura

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The distribution of collagen types I, III, and IV and of laminin, fibronectin, and keratin was studied in otitis media experimentally induced by Streptococcus pneumoniae in the chinchilla. The expression of interstitial collagen types I and III and of fibronectin was increased in the subepithelial space that was thickened by inflammation in the acute period of infection. The expression of collagen type IV in the subepithelial space could be seen in the early period. The epithelial cells in the middle ear changed from flat cuboidal to pseudostratified columnar in pneumococcus- inoculated ears, and the number of keratin-positive epithelial cells in the middle ear increased remarkably after infection. These results indicate that changes in epithelial cell differentiation effected by the extracellular matrix correlate with changes in expression of keratin. It is proposed that the extracellular matrix may contribute to tissue repair in the middle ear after bacterial infection by interfering with cell proliferation of epithelial cells and fibroblasts.

Original languageEnglish (US)
Pages (from-to)769-776
Number of pages8
JournalAnnals of Otology, Rhinology and Laryngology
Volume108
Issue number8
DOIs
StatePublished - Jan 1 1999

Keywords

  • Extracellular matrix
  • Immunohistochemistry
  • Keratin
  • Otitis media
  • Streptococcus pneumoniae

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