Immunohistochemical assessment of proliferation markers and altered gene expression in archival specimens of ovarian epithelial tumors

M. A. Khalifa, D. A. Lacher, J. M. Lage, R. S. Mannel, J. L. Walker, L. H. Angros, K. W. Min

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Recently reported morphologic end molecular genetic evidence suggests that some ovarian carcinomas arise from their benign end low malignant potential (IMP) counterparts. In order to help reach a better understanding of ovarian tumorigenesis, we studied a wide range of gene products involved in cellular growth regulation in archival material obtained from three groups of tumors with graduated malignant potential. Immunohistochemical staining was performed for Ki-67, proliferating cell nuclear antigen (PCNA), epidermal growth factor receptor (EGFR), HER-2/neu-encoded receptor protein, p53 gene product, end multidrug resistance gene product (P-glycoprotein). The expression of EGFR, HER-2/neu-encoded receptor protein, end mutant p53 product was significantly lower in LMP tumors than in carcinomas (p < 0.05). HER-2/neu immunopositivity was more prevalent in adenocarcinomas then in LMP tumors, and the proportion of HER-2/neu-positive adenocarcinomas increased with the progression of the disease. The staining differences between LMP tumors and adenocarcinomas with antibodies against Ki-67, PCNA, and P-glycoprotein were not statistically significant. Immunohistochemical detection of EGFR, HER-2/neu, and p53 in ovarian epithelial tumor is relevant to ovarian tumorigenesis. It could serve as a powerful tool for the pursuit of retrospective studies focused on these important biologic markers.

Original languageEnglish (US)
Pages (from-to)532-539
Number of pages8
JournalCancer Detection and Prevention
Volume21
Issue number6
StatePublished - 1997

Keywords

  • Borderline tumors
  • Low malignant potential tumors
  • Oncoproteins
  • Ovarian neoplasms
  • Proliferation markers
  • Protooncogenes

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