Immunohistochemical analysis of insulin-like growth factor-binding proteins -1, -2, and-3 in implantation sites of the mouse

Mark A Damario, Hung Ching Liu, Carol Ann Mele, Marcelo G. Horenstein, Zev Rosenwaks

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Purpose: Our purpose was to analyze potential interactions between the embryo and the maternal endometrial interface in vivo by analyzing immunolocalization of insulin-like growth factor-binding proteins (IGFBPs) - 1, -2, and -3 in implantation sites of the mouse. Methods: Six-week-old B6D2F1 female mice underwent superovulation followed by mating and sacrifice at timed intervals. Formalin-fixed paraffin-embedded tissue was used for avidin-biotin immunocytochemical localization of IGFBPs utilizing standard methodology. Results: Immunostaining at 1.5 days post coitum revealed light staining in the epithelial glandular cells and faint staining in decidual stroma for both IGFBP-1 and IGFBP-2. At 7.5-10.5 days post coitum, there was moderate-dense immunostaining in the decidualized stromal cells at the implantation site for all three IGFBPs, whereas light immunostaining was seen in nonimplantation site decidua. Conclusions: Compartmentalization of immunostaining for IGFBP-1, -2, and -3 within decidualized stroma suggests that these proteins may be regulated by trophoblastic and/or embryonic signals.

Original languageEnglish (US)
Pages (from-to)513-520
Number of pages8
JournalJournal of Assisted Reproduction and Genetics
Issue number8
StatePublished - 1998


  • Decidua
  • Immunohistochemistry
  • Implantation
  • Insulin-like growth factor- binding proteins


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