Immunogold labeling of Alzheimer paired helical filaments with ganglioside mab A2B5

J. R. Clements, A. J. Beitz, C. R. Emory, W. H. Frey

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The ganglioside monoclonal antibody A2B5 has previously been used at the light microscopic level to label Alzheimer neurofibrillary tangles (NFTs). Light microscopic analysis, however, could not reveal whether the A2B5 antibody-labeled NFTs or membrane fragments associated with NFTs. Therefore, we used pre-embedding immunohistochemical electron microscopy to examine A2B5 labeling of NFT. We found that the A2B5 antibody does indeed label a NFT antigen associated with the paired helical filament (PHF) structure, while no significant labeling of membranes or membrane fragments was observed. However, no clear periodicity of the immunogold label on the PHF was found.

Original languageEnglish (US)
Pages (from-to)35-42
Number of pages8
JournalAlzheimer disease and associated disorders
Volume4
Issue number1
DOIs
StatePublished - 1990

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