Immunocytochemical localization of TNF type 1 and type 2 receptors in the rat spinal cord

Gregory M. Holmes, Sadie L. Hebert, Richard C. Rogers, Gerlinda E. Hermann

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Tumor necrosis factor-α (TNF-α) is secreted in numerous pathophysiological situations by a variety of cell types. Tactile hypersensitivity (allodynia) is one component of a constellation of "illness behaviors" triggered by TNF-α. TNF-α is also implicated in neuropathic pain after peripheral nerve injury and apoptosis after spinal cord injury (SCI). It is possible that SCI, illness- and peripheral injury-induced hypersensitivity may share a similar spinal mediated etiology. These studies identify the locus of type-1 TNF (TNFR1 or p55) and type-2 TNF (TNFR2 or p75) receptors within the spinal cord. At all spinal levels, TNFR1 receptor immunoreactivity (TNFR1-ir) was constitutively expressed on cells and afferent fibers within the dorsal root ganglia, afferent fibers of the dorsal root, dorsal root entry zone (REZ) and within lamina I and II of the dorsal horn. Unilateral dorsal rhizotomy eliminated the characteristic pattern of TNFR1-ir at the rhizotomized REZ. In contrast, TNFR2-ir was consistently absent from dorsal root fibers and the region of the root entry zone. Consistent with our previous report, medullary afferent fibers in the solitary tract and spinal trigeminal tract labelled for TNF1-ir, but did not express TNFR2-ir. The presence TNFR1-ir on dorsal horn afferents, suggests that TNF-α may be a mechanism responsible for tactile hypersensitivity during illness. The presence of TNFR1 receptors, and perhaps their long-term activation or plasticity, may also play a critical role in the chronic allodynia and hyperreflexia observed after SCI or peripheral nerve damage.

Original languageEnglish (US)
Pages (from-to)210-219
Number of pages10
JournalBrain Research
Volume1025
Issue number1-2
DOIs
StatePublished - Oct 29 2004

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Receptors, Tumor Necrosis Factor, Type I
Receptors, Tumor Necrosis Factor, Type II
Spinal Cord
Spinal Nerve Roots
Tumor Necrosis Factor-alpha
Spinal Cord Injuries
Hypersensitivity
Hyperalgesia
Substantia Gelatinosa
Rhizotomy
Illness Behavior
Peripheral Nerve Injuries
Abnormal Reflexes
Touch
Spinal Ganglia
Neuralgia
Peripheral Nerves
Apoptosis
Wounds and Injuries
Spinal Cord Dorsal Horn

Keywords

  • Allodynia
  • Cytokine
  • Illness behavior
  • Immune-neural interaction
  • Neural injury
  • Nociception

Cite this

Immunocytochemical localization of TNF type 1 and type 2 receptors in the rat spinal cord. / Holmes, Gregory M.; Hebert, Sadie L.; Rogers, Richard C.; Hermann, Gerlinda E.

In: Brain Research, Vol. 1025, No. 1-2, 29.10.2004, p. 210-219.

Research output: Contribution to journalArticle

Holmes, Gregory M. ; Hebert, Sadie L. ; Rogers, Richard C. ; Hermann, Gerlinda E. / Immunocytochemical localization of TNF type 1 and type 2 receptors in the rat spinal cord. In: Brain Research. 2004 ; Vol. 1025, No. 1-2. pp. 210-219.
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