Immunocytochemical localization of glutamate dehydrogenase in mitochondria of the cerebellum: an ultrastructural study using a monoclonal antibody

J. E. Madl, J. R. Clements, Al J Beitz, R. J. Wenthold, Alice A Larson

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17 Scopus citations

Abstract

Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degress of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and astrocytes in the cerebellum of the rat. Differential intensity of staining among mitochondria within individual glial cells and between glial cells was observed and may provide an anatomical means of detecting differences in glutamate metabolism.

Original languageEnglish (US)
Pages (from-to)396-402
Number of pages7
JournalBrain Research
Volume452
Issue number1-2
DOIs
StatePublished - Jun 14 1988

Keywords

  • Glia
  • Glutamate
  • Glutamate dehydrogenase
  • Mitochondrion

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