Immunocytochemical localization of glutamate dehydrogenase in mitochondria of the cerebellum: an ultrastructural study using a monoclonal antibody

J. E. Madl; J. R. Clements; Al J Beitz; R. J. Wenthold; Alice A Larson

doi:10.1016/0006-8993(88)90047-9

Immunocytochemical localization of glutamate dehydrogenase in mitochondria of the cerebellum: an ultrastructural study using a monoclonal antibody

J. E. Madl, J. R. Clements, Al J Beitz, R. J. Wenthold, Alice A Larson

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degress of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and astrocytes in the cerebellum of the rat. Differential intensity of staining among mitochondria within individual glial cells and between glial cells was observed and may provide an anatomical means of detecting differences in glutamate metabolism.

Original language	English (US)
Pages (from-to)	396-402
Number of pages	7
Journal	Brain Research
Volume	452
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(88)90047-9
State	Published - Jun 14 1988

Keywords

Glia
Glutamate
Glutamate dehydrogenase
Mitochondrion

Publisher link

10.1016/0006-8993(88)90047-9

Find It

Find It at U of M Libraries

Cite this

@article{3028784ef1a746bca543616c9cec29bc,

title = "Immunocytochemical localization of glutamate dehydrogenase in mitochondria of the cerebellum: an ultrastructural study using a monoclonal antibody",

abstract = "Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degress of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and astrocytes in the cerebellum of the rat. Differential intensity of staining among mitochondria within individual glial cells and between glial cells was observed and may provide an anatomical means of detecting differences in glutamate metabolism.",

keywords = "Glia, Glutamate, Glutamate dehydrogenase, Mitochondrion",

author = "Madl, {J. E.} and Clements, {J. R.} and Beitz, {Al J} and Wenthold, {R. J.} and Larson, {Alice A}",

year = "1988",

month = jun,

day = "14",

doi = "10.1016/0006-8993(88)90047-9",

language = "English (US)",

volume = "452",

pages = "396--402",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Immunocytochemical localization of glutamate dehydrogenase in mitochondria of the cerebellum

T2 - an ultrastructural study using a monoclonal antibody

AU - Madl, J. E.

AU - Clements, J. R.

AU - Beitz, Al J

AU - Wenthold, R. J.

AU - Larson, Alice A

PY - 1988/6/14

Y1 - 1988/6/14

N2 - Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degress of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and astrocytes in the cerebellum of the rat. Differential intensity of staining among mitochondria within individual glial cells and between glial cells was observed and may provide an anatomical means of detecting differences in glutamate metabolism.

AB - Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degress of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and astrocytes in the cerebellum of the rat. Differential intensity of staining among mitochondria within individual glial cells and between glial cells was observed and may provide an anatomical means of detecting differences in glutamate metabolism.

KW - Glia

KW - Glutamate

KW - Glutamate dehydrogenase

KW - Mitochondrion

UR - http://www.scopus.com/inward/record.url?scp=0023923289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023923289&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(88)90047-9

DO - 10.1016/0006-8993(88)90047-9

M3 - Article

C2 - 3401748

AN - SCOPUS:0023923289

SN - 0006-8993

VL - 452

SP - 396

EP - 402

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Immunocytochemical localization of glutamate dehydrogenase in mitochondria of the cerebellum: an ultrastructural study using a monoclonal antibody

Abstract

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this