Immunocytochemical identification of laticifers

K. J. Wilson; B. H. Petersen; D. D. Biesboer

doi:10.1007/BF01279440

Immunocytochemical identification of laticifers

K. J. Wilson, B. H. Petersen, D. D. Biesboer

Center for Transportation Studies

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Abstract

sensitive immunocytochemical method for the identification of laticifers has been developed. Frozen sections of various laticifer-bearing plant material, mounted on slides, were first flooded with the IgG fraction of rabbit anti-latex antiserum, prepared using whole latex of Asclepias syriaca, then flooded with fluorescein-conjugated IgG fraction goat anti-rabbit IgG to visualize laticifers. Positive fluorescence was observed for laticifers in shoots and embryos of A. syriaca and Stapelia bella and embryos of A. tuberosa. Laticifers did not fluoresce in shoots of A. tuberosa and Euphorbia tirucalli, in embryos of E. marginata, or in petioles of Musa paradisiaca and Cichorium intybus. Controls prepared with uninjected rabbit serum were negative (no fluorescence).

Original language	English (US)
Pages (from-to)	86-90
Number of pages	5
Journal	Protoplasma
Volume	122
Issue number	1-2
DOIs	https://doi.org/10.1007/BF01279440
State	Published - Feb 1984

Keywords

Articulated laticifers
Asclepiadaceae
Immunocytochemistry
Non-articulated laticifers

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10.1007/BF01279440

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title = "Immunocytochemical identification of laticifers",

abstract = "sensitive immunocytochemical method for the identification of laticifers has been developed. Frozen sections of various laticifer-bearing plant material, mounted on slides, were first flooded with the IgG fraction of rabbit anti-latex antiserum, prepared using whole latex of Asclepias syriaca, then flooded with fluorescein-conjugated IgG fraction goat anti-rabbit IgG to visualize laticifers. Positive fluorescence was observed for laticifers in shoots and embryos of A. syriaca and Stapelia bella and embryos of A. tuberosa. Laticifers did not fluoresce in shoots of A. tuberosa and Euphorbia tirucalli, in embryos of E. marginata, or in petioles of Musa paradisiaca and Cichorium intybus. Controls prepared with uninjected rabbit serum were negative (no fluorescence).",

keywords = "Articulated laticifers, Asclepiadaceae, Immunocytochemistry, Non-articulated laticifers",

author = "Wilson, {K. J.} and Petersen, {B. H.} and Biesboer, {D. D.}",

year = "1984",

month = feb,

doi = "10.1007/BF01279440",

language = "English (US)",

volume = "122",

pages = "86--90",

journal = "Protoplasma",

issn = "0033-183X",

publisher = "Springer Wien",

number = "1-2",

}

TY - JOUR

T1 - Immunocytochemical identification of laticifers

AU - Wilson, K. J.

AU - Petersen, B. H.

AU - Biesboer, D. D.

PY - 1984/2

Y1 - 1984/2

N2 - sensitive immunocytochemical method for the identification of laticifers has been developed. Frozen sections of various laticifer-bearing plant material, mounted on slides, were first flooded with the IgG fraction of rabbit anti-latex antiserum, prepared using whole latex of Asclepias syriaca, then flooded with fluorescein-conjugated IgG fraction goat anti-rabbit IgG to visualize laticifers. Positive fluorescence was observed for laticifers in shoots and embryos of A. syriaca and Stapelia bella and embryos of A. tuberosa. Laticifers did not fluoresce in shoots of A. tuberosa and Euphorbia tirucalli, in embryos of E. marginata, or in petioles of Musa paradisiaca and Cichorium intybus. Controls prepared with uninjected rabbit serum were negative (no fluorescence).

AB - sensitive immunocytochemical method for the identification of laticifers has been developed. Frozen sections of various laticifer-bearing plant material, mounted on slides, were first flooded with the IgG fraction of rabbit anti-latex antiserum, prepared using whole latex of Asclepias syriaca, then flooded with fluorescein-conjugated IgG fraction goat anti-rabbit IgG to visualize laticifers. Positive fluorescence was observed for laticifers in shoots and embryos of A. syriaca and Stapelia bella and embryos of A. tuberosa. Laticifers did not fluoresce in shoots of A. tuberosa and Euphorbia tirucalli, in embryos of E. marginata, or in petioles of Musa paradisiaca and Cichorium intybus. Controls prepared with uninjected rabbit serum were negative (no fluorescence).

KW - Articulated laticifers

KW - Asclepiadaceae

KW - Immunocytochemistry

KW - Non-articulated laticifers

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DO - 10.1007/BF01279440

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VL - 122

SP - 86

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JO - Protoplasma

JF - Protoplasma

IS - 1-2

ER -

Immunocytochemical identification of laticifers

Abstract

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