sensitive immunocytochemical method for the identification of laticifers has been developed. Frozen sections of various laticifer-bearing plant material, mounted on slides, were first flooded with the IgG fraction of rabbit anti-latex antiserum, prepared using whole latex of Asclepias syriaca, then flooded with fluorescein-conjugated IgG fraction goat anti-rabbit IgG to visualize laticifers. Positive fluorescence was observed for laticifers in shoots and embryos of A. syriaca and Stapelia bella and embryos of A. tuberosa. Laticifers did not fluoresce in shoots of A. tuberosa and Euphorbia tirucalli, in embryos of E. marginata, or in petioles of Musa paradisiaca and Cichorium intybus. Controls prepared with uninjected rabbit serum were negative (no fluorescence).
- Articulated laticifers
- Non-articulated laticifers