Immunocytochemical evidence for particulate localization of phenylethanolamine-N-methyltransferase in adrenal medulla

L. S. Van Orden, J. P. Burke, Jan A. Redick, K. E. Rybarczyk, Dianna E. Van Orden, Harriet A. Baker, B. K. Hartman

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Abstract

Previous biochemical evidence for the localization of phenylethanolamine-N-methyltransferase in adrenal medulla has indicated that this enzyme is soluble, although some workers have reported a significant amount of particulate phenylethanolamine-N-methyltransferase (Joh and Goldstein, 1973). Electron microscopic immunocytochemical study of bovine adrenal medulla has revealed a distinct particulate localization of phenylethanolamine-N-methyltransferase in association with chromaffin granules. It is therefore suggested that some, and possibly most, adrenal phenylethanolamine-N-methyltransferase may be in a particulate form in intact tissue, and that solubilization artifacts during tissue preparation could account in part for the large proportion of soluble phenylethanolarmine-N-methyltransferase reported previously.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalNeuropharmacology
Volume16
Issue number2
DOIs
StatePublished - Feb 1977

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    Van Orden, L. S., Burke, J. P., Redick, J. A., Rybarczyk, K. E., Van Orden, D. E., Baker, H. A., & Hartman, B. K. (1977). Immunocytochemical evidence for particulate localization of phenylethanolamine-N-methyltransferase in adrenal medulla. Neuropharmacology, 16(2), 129-133. https://doi.org/10.1016/0028-3908(77)90060-0