Immunochemical studies of phospholipids: Production of antibodies to triphosphoinositide

A. J. Greenberg, A. J. Trevor, D. A. Johnson, H. H. Loh

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Antibodies to triphosphoinositide (TPI, I-phosphatidylinositol-3,4-bisphosphate) were raised in rabbits immunized with a hapten suspension of TPI, lecithin and cholesterol (1:4:30 by wt) in 0.1% methylated BSA. The hapten suspension was administered i.v. every two days for three weeks with each rabbit receiving a total of 3 mg TPI. Immunodiffusion with the partially purified immunoglobulin fraction and the comparison of complement-isofixation curves demonstrate complete cross-reactivity between TPI and diphosphoinositide (DPI) and very little specificity towards phosphatidylinositol (PI) and cardiolipin.

Original languageEnglish (US)
Pages (from-to)193-196
Number of pages4
JournalMolecular Immunology
Issue number3
StatePublished - Mar 1979

Bibliographical note

Funding Information:
*This is publication No. 78-22 from the Department of Pharmacology, University of California, San Francisco, CA. t This work was supported in part by USPHS NIDA Grant DA-01583. $A. J. Greenberg is a postdoctoral USPHS NIDA Grant DA-00006 # IUPAC-IUB commission recommendations, published in Lipid.~ (1977) 12, 455.


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