Immunochemical studies of phospholipids: Production of antibodies to triphosphoinositide

A. J. Greenberg, A. J. Trevor, D. A. Johnson, H. H. Loh

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Antibodies to triphosphoinositide (TPI, I-phosphatidylinositol-3,4-bisphosphate) were raised in rabbits immunized with a hapten suspension of TPI, lecithin and cholesterol (1:4:30 by wt) in 0.1% methylated BSA. The hapten suspension was administered i.v. every two days for three weeks with each rabbit receiving a total of 3 mg TPI. Immunodiffusion with the partially purified immunoglobulin fraction and the comparison of complement-isofixation curves demonstrate complete cross-reactivity between TPI and diphosphoinositide (DPI) and very little specificity towards phosphatidylinositol (PI) and cardiolipin.

Original languageEnglish (US)
Pages (from-to)193-196
Number of pages4
JournalMolecular Immunology
Issue number3
StatePublished - Mar 1979

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