Antibodies to triphosphoinositide (TPI, I-phosphatidylinositol-3,4-bisphosphate) were raised in rabbits immunized with a hapten suspension of TPI, lecithin and cholesterol (1:4:30 by wt) in 0.1% methylated BSA. The hapten suspension was administered i.v. every two days for three weeks with each rabbit receiving a total of 3 mg TPI. Immunodiffusion with the partially purified immunoglobulin fraction and the comparison of complement-isofixation curves demonstrate complete cross-reactivity between TPI and diphosphoinositide (DPI) and very little specificity towards phosphatidylinositol (PI) and cardiolipin.
Bibliographical noteFunding Information:
*This is publication No. 78-22 from the Department of Pharmacology, University of California, San Francisco, CA. t This work was supported in part by USPHS NIDA Grant DA-01583. $A. J. Greenberg is a postdoctoral USPHS NIDA Grant DA-00006 # IUPAC-IUB commission recommendations, published in Lipid.~ (1977) 12, 455.