Immunochemical studies of phospholipids: Production of antibodies to triphosphoinositide

A. J. Greenberg; A. J. Trevor; D. A. Johnson; H. H. Loh

doi:10.1016/0161-5890(79)90145-7

Immunochemical studies of phospholipids: Production of antibodies to triphosphoinositide

A. J. Greenberg, A. J. Trevor, D. A. Johnson, H. H. Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Antibodies to triphosphoinositide (TPI, I-phosphatidylinositol-3,4-bisphosphate) were raised in rabbits immunized with a hapten suspension of TPI, lecithin and cholesterol (1:4:30 by wt) in 0.1% methylated BSA. The hapten suspension was administered i.v. every two days for three weeks with each rabbit receiving a total of 3 mg TPI. Immunodiffusion with the partially purified immunoglobulin fraction and the comparison of complement-isofixation curves demonstrate complete cross-reactivity between TPI and diphosphoinositide (DPI) and very little specificity towards phosphatidylinositol (PI) and cardiolipin.

Original language	English (US)
Pages (from-to)	193-196
Number of pages	4
Journal	Molecular Immunology
Volume	16
Issue number	3
DOIs	https://doi.org/10.1016/0161-5890(79)90145-7
State	Published - Mar 1979

Bibliographical note

Funding Information:
*This is publication No. 78-22 from the Department of Pharmacology, University of California, San Francisco, CA. t This work was supported in part by USPHS NIDA Grant DA-01583. $A. J. Greenberg is a postdoctoral USPHS NIDA Grant DA-00006 # IUPAC-IUB commission recommendations, published in Lipid.~ (1977) 12, 455.

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@article{f01e32da1a5042e6bde1f10fad4603e6,

title = "Immunochemical studies of phospholipids: Production of antibodies to triphosphoinositide",

abstract = "Antibodies to triphosphoinositide (TPI, I-phosphatidylinositol-3,4-bisphosphate) were raised in rabbits immunized with a hapten suspension of TPI, lecithin and cholesterol (1:4:30 by wt) in 0.1% methylated BSA. The hapten suspension was administered i.v. every two days for three weeks with each rabbit receiving a total of 3 mg TPI. Immunodiffusion with the partially purified immunoglobulin fraction and the comparison of complement-isofixation curves demonstrate complete cross-reactivity between TPI and diphosphoinositide (DPI) and very little specificity towards phosphatidylinositol (PI) and cardiolipin.",

author = "Greenberg, {A. J.} and Trevor, {A. J.} and Johnson, {D. A.} and Loh, {H. H.}",

note = "Funding Information: *This is publication No. 78-22 from the Department of Pharmacology, University of California, San Francisco, CA. t This work was supported in part by USPHS NIDA Grant DA-01583. $A. J. Greenberg is a postdoctoral USPHS NIDA Grant DA-00006 # IUPAC-IUB commission recommendations, published in Lipid.~ (1977) 12, 455.",

year = "1979",

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doi = "10.1016/0161-5890(79)90145-7",

language = "English (US)",

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pages = "193--196",

journal = "Immunochemistry",

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TY - JOUR

T1 - Immunochemical studies of phospholipids

T2 - Production of antibodies to triphosphoinositide

AU - Greenberg, A. J.

AU - Trevor, A. J.

AU - Johnson, D. A.

AU - Loh, H. H.

N1 - Funding Information: *This is publication No. 78-22 from the Department of Pharmacology, University of California, San Francisco, CA. t This work was supported in part by USPHS NIDA Grant DA-01583. $A. J. Greenberg is a postdoctoral USPHS NIDA Grant DA-00006 # IUPAC-IUB commission recommendations, published in Lipid.~ (1977) 12, 455.

PY - 1979/3

Y1 - 1979/3

N2 - Antibodies to triphosphoinositide (TPI, I-phosphatidylinositol-3,4-bisphosphate) were raised in rabbits immunized with a hapten suspension of TPI, lecithin and cholesterol (1:4:30 by wt) in 0.1% methylated BSA. The hapten suspension was administered i.v. every two days for three weeks with each rabbit receiving a total of 3 mg TPI. Immunodiffusion with the partially purified immunoglobulin fraction and the comparison of complement-isofixation curves demonstrate complete cross-reactivity between TPI and diphosphoinositide (DPI) and very little specificity towards phosphatidylinositol (PI) and cardiolipin.

AB - Antibodies to triphosphoinositide (TPI, I-phosphatidylinositol-3,4-bisphosphate) were raised in rabbits immunized with a hapten suspension of TPI, lecithin and cholesterol (1:4:30 by wt) in 0.1% methylated BSA. The hapten suspension was administered i.v. every two days for three weeks with each rabbit receiving a total of 3 mg TPI. Immunodiffusion with the partially purified immunoglobulin fraction and the comparison of complement-isofixation curves demonstrate complete cross-reactivity between TPI and diphosphoinositide (DPI) and very little specificity towards phosphatidylinositol (PI) and cardiolipin.

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U2 - 10.1016/0161-5890(79)90145-7

DO - 10.1016/0161-5890(79)90145-7

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AN - SCOPUS:0018387214

SN - 0161-5890

VL - 16

SP - 193

EP - 196

JO - Immunochemistry

JF - Immunochemistry

IS - 3

ER -

Immunochemical studies of phospholipids: Production of antibodies to triphosphoinositide

Abstract

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