Immunoaffinity purification of bovine factor VII

R. Bach, J. Oberdick, Y. Nemerson

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Factor VII has been purified to homogeneity from bovine plasma by a procedure that includes affinity purification on an immunoadsorbent column. Recovery was determined by both coagulant assay and liguid scintillation counting, using 3H-factor VII as an interval standard. The purification factor calculated by both methods was ~ 120,000-fold, with a final yield of ~ 18%. Homogeneity was assessed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The material migrated as a single polypeptide chain of 53,000 daltons, and following activation by factor Xa, the one-chain zymogen was quantitatively converted to two-chain factor VIIa. Conversion of affinity-purified factor VII to factor VIIa resulted in up to a 119-fold activation of the coagulation activity, which is 2.7-4 times greater than the activatability reported for factor VII prepared by other methods. Zur et al. calculated that pure factor VII, uncontamination by traces of factor VIIa, would be activated 123-fold upon conversion to factor VIIa. The close agreement between observed activatability of affinity-purified factor VII and the theoretical prediction suggests that we have isolated factor VII essentially free of factor VIIa. The purification data from three lots of bovine plasma yield an estimate for the plasma concentration of factor VII from 10.1 nM to 18.5 nM.

Original languageEnglish (US)
Pages (from-to)393-398
Number of pages6
Issue number2
StatePublished - 1984


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