Immunoaffinity purification: Basic principles and operational considerations

M. L. Yarmush, A. M. Weiss, K. P. Antonsen, D. J. Odde, D. M. Yarmush

Research output: Contribution to journalReview article

36 Scopus citations

Abstract

Immunoaffinity purification has become an important technique in biotechnology. In this review the basic principles of immunoaffinity separations are described with respect to the stages of operation and potential application. The most commonly used support materials, activation procedures, and coupling chemistries are compared to one another for suitability in various applications. Individual operational steps for fixed bed immunoadsorbents including loading, washing, elution and regeneration are described in terms of both theory and practice. Factors influencing adsorbent stability are identified, and alternative operation and configuration strategies are discussed in light of their application to immunoaffinity systems.

Original languageEnglish (US)
Pages (from-to)413-446
Number of pages34
JournalBiotechnology Advances
Volume10
Issue number3
DOIs
StatePublished - 1992

Keywords

  • Immunoaffinity purification
  • affinity adsorption
  • affinity chromatography
  • immobilization
  • immunoaffinity chromatography
  • monoclonal antibodies

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