Immunization of mice with the antigen A60 oi Mycobacterium bovis BCG

R. D. HUBBARD, C. M. FLORY, F. M. COLLINS, C. COCITO

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Antigen 60 (A60) is a thermostable component of the cytoplasm of Mycobacterium tuberculosis and BCG which can be fractionated into at least 15 protein bands when analysed by Western blot. Normal B6D2 mice were immunized subcutaneously with 20 μg of the A60 protein suspended in Freund's incomplete adjuvant (FIA) or in saline. Three weeks later the mice received a second dose of vaccine followed 2 weeks later by an aerogenic challenge with approximately 103 CFU of M. tuberculosis Erdman. The mice receiving the adjuvanled A60 showed a significant reduction (P < 0·05) in the number of viable organisms recovered from the lungs and the spleen 3 weeks after challenge. However, this response was less than that seen in BCG vaccinated controls.

Original languageEnglish (US)
Pages (from-to)129-131
Number of pages3
JournalClinical & Experimental Immunology
Volume88
Issue number1
DOIs
StatePublished - Apr 1992

Keywords

  • BCG
  • antigen A60
  • mice
  • protection

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