TY - JOUR
T1 - Immune response to ORF5a protein immunization is not protective against porcine reproductive and respiratory syndrome virus infection
AU - Robinson, Sally R.
AU - Figueiredo, Marina C.
AU - Abrahante, Juan E.
AU - Murtaugh, Michael P.
N1 - Funding Information:
SRR was supported by NIH Comparative Medicine and Pathology training grant T32RR018719 .
PY - 2013
Y1 - 2013
N2 - Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped RNA virus responsible for PRRS in swine, a disease with globally significant animal welfare and economic concerns. There is no specific treatment and variably effective immune protection. Molecular mechanisms responsible for virulence, pathogenesis and protective immune response remain poorly understood. These factors limit progress toward development of effective measures for prevention and treatment of PRRS. A novel PRRSV ORF5a protein, encoded in an open reading frame (ORF) that overlaps the major envelope glycoprotein GP5 ORF, was recently identified. Because ORF5a is highly conserved in diverse PRRSV isolates, is a structural protein in the virion, and elicits a specific antibody response in infected pigs, we investigated its potential role in immune protection against PRRSV infection. Pigs immunized with ORF5a protein had robust serologic responses. However, the antibodies did not neutralize virus, and immunity did not protect against challenge infection. We conclude from these findings that the ORF5a antibody response is neither neutralizing nor protective.
AB - Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped RNA virus responsible for PRRS in swine, a disease with globally significant animal welfare and economic concerns. There is no specific treatment and variably effective immune protection. Molecular mechanisms responsible for virulence, pathogenesis and protective immune response remain poorly understood. These factors limit progress toward development of effective measures for prevention and treatment of PRRS. A novel PRRSV ORF5a protein, encoded in an open reading frame (ORF) that overlaps the major envelope glycoprotein GP5 ORF, was recently identified. Because ORF5a is highly conserved in diverse PRRSV isolates, is a structural protein in the virion, and elicits a specific antibody response in infected pigs, we investigated its potential role in immune protection against PRRSV infection. Pigs immunized with ORF5a protein had robust serologic responses. However, the antibodies did not neutralize virus, and immunity did not protect against challenge infection. We conclude from these findings that the ORF5a antibody response is neither neutralizing nor protective.
KW - Arterivirus
KW - Immunology
KW - Neutralizing antibody
KW - PRRSV
KW - Swine
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U2 - 10.1016/j.vetmic.2013.03.006
DO - 10.1016/j.vetmic.2013.03.006
M3 - Article
C2 - 23578707
AN - SCOPUS:84877132487
SN - 0378-1135
VL - 164
SP - 281
EP - 285
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -