Immediate consequences of cigarette smoking: Rapid formation of polycyclic aromatic hydrocarbon diol epoxides

Yan Zhong, Steven G. Carmella, Pramod Upadhyaya, J. Bradley Hochalter, Diane Rauch, Andrew Oliver, Joni Jensen, Dorothy Hatsukami, Jing Wang, Cheryl Zimmerman, Stephen S. Hecht

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Polycyclic aromatic hydrocarbons (PAH) are among the likely major causative agents for lung cancer in smokers. PAH require metabolic activation to exert their carcinogenic effects, and one important pathway proceeds through a three-step sequence resulting in the formation of diol epoxides, which react with DNA to produce adducts that can cause mutations and initiate the carcinogenic process. However, no previous published studies have examined this critical pathway in humans specifically exposed to PAH by inhalation of cigarette smoke. This study used a unique approach employing a stable isotope derivative of phenanthrene, the simplest PAH with a bay region, a feature closely associated with PAH carcinogenicity. Twelve subjects each smoked a cigarette to which [D10]phenanthrene had been added. Plasma was analyzed for [D10]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4- tetrahydrophenanthrene ([D10]PheT), the major end product of the diol epoxide metabolism pathway of phenanthrene. The analysis was performed by gas chromatography-negative ion chemical ionization-tandem mass spectrometry, using [13C6]PheT as internal standard. The results demonstrated that the three-step pathway resulting in the formation of diol epoxides, as monitored by [D10]PheT, occurred with remarkable rapidity. Levels of [D10]PheT in plasma of all subjects were maximal at the earliest time points examined, 15-30 min after smoking the cigarette containing [D 10]phenanthrene, and decreased thereafter. These results demonstrate that the formation of a PAH diol epoxide occurs rapidly in smokers. Because PAH diol epoxides are mutagenic and carcinogenic, the results clearly demonstrate immediate negative health consequences of smoking, which should serve as a major warning to anyone contemplating initiating tobacco use.

Original languageEnglish (US)
Pages (from-to)246-252
Number of pages7
JournalChemical research in toxicology
Volume24
Issue number2
DOIs
StatePublished - Feb 18 2011

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