Imaging the vascular response to inhaled vasoactive compounds

Kenneth T. Kotz; Khalid Amin; Juan M. Orduna; Gregory W. Faris

Imaging the vascular response to inhaled vasoactive compounds

Kenneth T. Kotz
, Khalid Amin
, Juan M. Orduna
, Gregory W. Faris

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have used a CW imaging apparatus to measure the near infrared (NIR) optical properties of rodents in vivo. We observed changes in the tissue vasculature of the animals in response to inspiratory hypercapnia (elevated CO₂) and hyperoxia (elevated O₂). The dynamics were dependent upon the inspired gas concentrations.

Original language	English (US)
Title of host publication	Frontiers in Optics, FiO-2004
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Electronic)	1557527792
State	Published - 2004
Externally published	Yes
Event	Frontiers in Optics, FiO-2004 - Rochester, United States Duration: Oct 12 2004 → …

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Conference

Conference	Frontiers in Optics, FiO-2004
Country/Territory	United States
City	Rochester
Period	10/12/04 → …

Bibliographical note

Funding Information:
We have chosen an animal model (nude mice with tumor xenografts), which allows the study of contrast during tumor progression. We partially immerse the animals in a matching medium (Ropaque and water, 37º C) in order to eliminate the non-uniformity in tissue thickness and geometry [2]. Near infrared (NIR) light from CW LEDs (780 nm and 840 nm) is passed through the sample and collected with a monochrome, digital CCD camera (Point Grey Research). Concentrations of oxygen and carbon dioxide are varied with respect to the surrounding ambient air via individual mass flow controllers and delivered to the mouse via a custom nose cone. The hardware is interfaced and controlled with a laptop computer. The collected images are post-processed using MATLAB and ImageJ. We observe dynamic changes in the optical properties of the rodents’ tissues in response to different levels of inspiratory hypercapnia and hyperoxia. The changes in response to gas inhalation follow similar trends observed with point-based measurements of cancerous tumors [3, 4]. The observed response at 780 nm was particularly sensitive to the O2gas concentration, while the 840 nm response was sensitive to the CO2 concentration (Fig. 1). Furthermore, the differential response enhanced the image contrast between the cancerous tissue and the surrounding tissues of the rodents. This project was funded by the DOD Breast Cancer Research Program, DAMD170920109570.

Publisher Copyright:
© 2004 OSA/FIO 2004

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title = "Imaging the vascular response to inhaled vasoactive compounds",

abstract = "We have used a CW imaging apparatus to measure the near infrared (NIR) optical properties of rodents in vivo. We observed changes in the tissue vasculature of the animals in response to inspiratory hypercapnia (elevated CO2) and hyperoxia (elevated O2). The dynamics were dependent upon the inspired gas concentrations.",

author = "Kotz, \{Kenneth T.\} and Khalid Amin and Orduna, \{Juan M.\} and Faris, \{Gregory W.\}",

note = "Funding Information: We have chosen an animal model (nude mice with tumor xenografts), which allows the study of contrast during tumor progression. We partially immerse the animals in a matching medium (Ropaque and water, 37º C) in order to eliminate the non-uniformity in tissue thickness and geometry [2]. Near infrared (NIR) light from CW LEDs (780 nm and 840 nm) is passed through the sample and collected with a monochrome, digital CCD camera (Point Grey Research). Concentrations of oxygen and carbon dioxide are varied with respect to the surrounding ambient air via individual mass flow controllers and delivered to the mouse via a custom nose cone. The hardware is interfaced and controlled with a laptop computer. The collected images are post-processed using MATLAB and ImageJ. We observe dynamic changes in the optical properties of the rodents{\textquoteright} tissues in response to different levels of inspiratory hypercapnia and hyperoxia. The changes in response to gas inhalation follow similar trends observed with point-based measurements of cancerous tumors [3, 4]. The observed response at 780 nm was particularly sensitive to the O2gas concentration, while the 840 nm response was sensitive to the CO2 concentration (Fig. 1). Furthermore, the differential response enhanced the image contrast between the cancerous tissue and the surrounding tissues of the rodents. This project was funded by the DOD Breast Cancer Research Program, DAMD170920109570. Publisher Copyright: {\textcopyright} 2004 OSA/FIO 2004; Frontiers in Optics, FiO-2004 ; Conference date: 12-10-2004",

year = "2004",

language = "English (US)",

series = "Optics InfoBase Conference Papers",

publisher = "Optica Publishing Group (formerly OSA)",

booktitle = "Frontiers in Optics, FiO-2004",

}

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T1 - Imaging the vascular response to inhaled vasoactive compounds

AU - Kotz, Kenneth T.

AU - Amin, Khalid

AU - Orduna, Juan M.

AU - Faris, Gregory W.

N1 - Funding Information: We have chosen an animal model (nude mice with tumor xenografts), which allows the study of contrast during tumor progression. We partially immerse the animals in a matching medium (Ropaque and water, 37º C) in order to eliminate the non-uniformity in tissue thickness and geometry [2]. Near infrared (NIR) light from CW LEDs (780 nm and 840 nm) is passed through the sample and collected with a monochrome, digital CCD camera (Point Grey Research). Concentrations of oxygen and carbon dioxide are varied with respect to the surrounding ambient air via individual mass flow controllers and delivered to the mouse via a custom nose cone. The hardware is interfaced and controlled with a laptop computer. The collected images are post-processed using MATLAB and ImageJ. We observe dynamic changes in the optical properties of the rodents’ tissues in response to different levels of inspiratory hypercapnia and hyperoxia. The changes in response to gas inhalation follow similar trends observed with point-based measurements of cancerous tumors [3, 4]. The observed response at 780 nm was particularly sensitive to the O2gas concentration, while the 840 nm response was sensitive to the CO2 concentration (Fig. 1). Furthermore, the differential response enhanced the image contrast between the cancerous tissue and the surrounding tissues of the rodents. This project was funded by the DOD Breast Cancer Research Program, DAMD170920109570. Publisher Copyright: © 2004 OSA/FIO 2004

PY - 2004

Y1 - 2004

N2 - We have used a CW imaging apparatus to measure the near infrared (NIR) optical properties of rodents in vivo. We observed changes in the tissue vasculature of the animals in response to inspiratory hypercapnia (elevated CO2) and hyperoxia (elevated O2). The dynamics were dependent upon the inspired gas concentrations.

AB - We have used a CW imaging apparatus to measure the near infrared (NIR) optical properties of rodents in vivo. We observed changes in the tissue vasculature of the animals in response to inspiratory hypercapnia (elevated CO2) and hyperoxia (elevated O2). The dynamics were dependent upon the inspired gas concentrations.

UR - https://www.scopus.com/pages/publications/85134728052

UR - https://www.scopus.com/pages/publications/85134728052#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85134728052

T3 - Optics InfoBase Conference Papers

BT - Frontiers in Optics, FiO-2004

PB - Optica Publishing Group (formerly OSA)

T2 - Frontiers in Optics, FiO-2004

Y2 - 12 October 2004

ER -

Imaging the vascular response to inhaled vasoactive compounds

Abstract

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