Imaging targets to identify chromosomal abnormalities in cells

S. Acevedo-Acevedo, B. Napiwocki, W. C. Crone

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Ensuring genetic stability in pluripotent stem cell (PSC) cultures is essential for the development of successful cell therapies. Many laboratories have found the emergence of genetic abnormalities in PSCs when cultured in vitro for prolonged amounts of time. These cells are mostly cultured in non-physiological stiff substrates like tissue culture polystyrene which produces the suspicion that the cause of these abnormalities may be influenced by substrate mechanics. In order to verify this, it is important to be able to determine and image, using fluorescence microscopy, potential targets within the cells that are indicative of genetic abnormalities. These genetic abnormalities are most likely to occur during cell division. Microtubules, comprised of the cytoskeletal protein tubulin, organize and separate chromosomes during cell division, thus it has been our main imaging target. We have been able to detect chromosomal abnormalities in human embryonic stem cells by fluorescence microscopy.

Original languageEnglish (US)
Title of host publicationMechanics of Biological Systems and Materials - Proceedings of the 2013 Annual Conference on Experimental and Applied Mechanics
Pages141-145
Number of pages5
DOIs
StatePublished - 2014
Externally publishedYes
Event2013 Annual Conference on Experimental and Applied Mechanics - Lombard, IL, United States
Duration: Jun 3 2013Jun 5 2013

Publication series

NameConference Proceedings of the Society for Experimental Mechanics Series
Volume4
ISSN (Print)2191-5644
ISSN (Electronic)2191-5652

Conference

Conference2013 Annual Conference on Experimental and Applied Mechanics
Country/TerritoryUnited States
CityLombard, IL
Period6/3/136/5/13

Bibliographical note

Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.

Keywords

  • Chromosomal abnormalities
  • Cytoskeleton
  • Immunofluorescence
  • Pluripotent stem cells
  • Substrate stiffness

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