Abstract
Ensuring genetic stability in pluripotent stem cell (PSC) cultures is essential for the development of successful cell therapies. Many laboratories have found the emergence of genetic abnormalities in PSCs when cultured in vitro for prolonged amounts of time. These cells are mostly cultured in non-physiological stiff substrates like tissue culture polystyrene which produces the suspicion that the cause of these abnormalities may be influenced by substrate mechanics. In order to verify this, it is important to be able to determine and image, using fluorescence microscopy, potential targets within the cells that are indicative of genetic abnormalities. These genetic abnormalities are most likely to occur during cell division. Microtubules, comprised of the cytoskeletal protein tubulin, organize and separate chromosomes during cell division, thus it has been our main imaging target. We have been able to detect chromosomal abnormalities in human embryonic stem cells by fluorescence microscopy.
Original language | English (US) |
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Title of host publication | Mechanics of Biological Systems and Materials - Proceedings of the 2013 Annual Conference on Experimental and Applied Mechanics |
Pages | 141-145 |
Number of pages | 5 |
DOIs | |
State | Published - 2014 |
Externally published | Yes |
Event | 2013 Annual Conference on Experimental and Applied Mechanics - Lombard, IL, United States Duration: Jun 3 2013 → Jun 5 2013 |
Publication series
Name | Conference Proceedings of the Society for Experimental Mechanics Series |
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Volume | 4 |
ISSN (Print) | 2191-5644 |
ISSN (Electronic) | 2191-5652 |
Conference
Conference | 2013 Annual Conference on Experimental and Applied Mechanics |
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Country/Territory | United States |
City | Lombard, IL |
Period | 6/3/13 → 6/5/13 |
Bibliographical note
Copyright:Copyright 2013 Elsevier B.V., All rights reserved.
Keywords
- Chromosomal abnormalities
- Cytoskeleton
- Immunofluorescence
- Pluripotent stem cells
- Substrate stiffness