Imaging targets to identify chromosomal abnormalities in cells

S. Acevedo-Acevedo; B. Napiwocki; W. C. Crone

doi:10.1007/978-3-319-00777-9_20

Imaging targets to identify chromosomal abnormalities in cells

S. Acevedo-Acevedo, B. Napiwocki, W. C. Crone

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Ensuring genetic stability in pluripotent stem cell (PSC) cultures is essential for the development of successful cell therapies. Many laboratories have found the emergence of genetic abnormalities in PSCs when cultured in vitro for prolonged amounts of time. These cells are mostly cultured in non-physiological stiff substrates like tissue culture polystyrene which produces the suspicion that the cause of these abnormalities may be influenced by substrate mechanics. In order to verify this, it is important to be able to determine and image, using fluorescence microscopy, potential targets within the cells that are indicative of genetic abnormalities. These genetic abnormalities are most likely to occur during cell division. Microtubules, comprised of the cytoskeletal protein tubulin, organize and separate chromosomes during cell division, thus it has been our main imaging target. We have been able to detect chromosomal abnormalities in human embryonic stem cells by fluorescence microscopy.

Original language	English (US)
Title of host publication	Mechanics of Biological Systems and Materials - Proceedings of the 2013 Annual Conference on Experimental and Applied Mechanics
Pages	141-145
Number of pages	5
DOIs	https://doi.org/10.1007/978-3-319-00777-9_20
State	Published - 2014
Externally published	Yes
Event	2013 Annual Conference on Experimental and Applied Mechanics - Lombard, IL, United States Duration: Jun 3 2013 → Jun 5 2013

Publication series

Name	Conference Proceedings of the Society for Experimental Mechanics Series
Volume	4
ISSN (Print)	2191-5644
ISSN (Electronic)	2191-5652

Conference

Conference	2013 Annual Conference on Experimental and Applied Mechanics
Country/Territory	United States
City	Lombard, IL
Period	6/3/13 → 6/5/13

Bibliographical note

Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.

Keywords

Chromosomal abnormalities
Cytoskeleton
Immunofluorescence
Pluripotent stem cells
Substrate stiffness

Publisher link

10.1007/978-3-319-00777-9_20

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Acevedo-Acevedo, S., Napiwocki, B., & Crone, W. C. (2014). Imaging targets to identify chromosomal abnormalities in cells. In Mechanics of Biological Systems and Materials - Proceedings of the 2013 Annual Conference on Experimental and Applied Mechanics (pp. 141-145). (Conference Proceedings of the Society for Experimental Mechanics Series; Vol. 4). https://doi.org/10.1007/978-3-319-00777-9_20

Imaging targets to identify chromosomal abnormalities in cells. / Acevedo-Acevedo, S.; Napiwocki, B.; Crone, W. C.

Mechanics of Biological Systems and Materials - Proceedings of the 2013 Annual Conference on Experimental and Applied Mechanics. 2014. p. 141-145 (Conference Proceedings of the Society for Experimental Mechanics Series; Vol. 4).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Acevedo-Acevedo, S, Napiwocki, B & Crone, WC 2014, Imaging targets to identify chromosomal abnormalities in cells. in Mechanics of Biological Systems and Materials - Proceedings of the 2013 Annual Conference on Experimental and Applied Mechanics. Conference Proceedings of the Society for Experimental Mechanics Series, vol. 4, pp. 141-145, 2013 Annual Conference on Experimental and Applied Mechanics, Lombard, IL, United States, 6/3/13. https://doi.org/10.1007/978-3-319-00777-9_20

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AU - Napiwocki, B.

AU - Crone, W. C.

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N2 - Ensuring genetic stability in pluripotent stem cell (PSC) cultures is essential for the development of successful cell therapies. Many laboratories have found the emergence of genetic abnormalities in PSCs when cultured in vitro for prolonged amounts of time. These cells are mostly cultured in non-physiological stiff substrates like tissue culture polystyrene which produces the suspicion that the cause of these abnormalities may be influenced by substrate mechanics. In order to verify this, it is important to be able to determine and image, using fluorescence microscopy, potential targets within the cells that are indicative of genetic abnormalities. These genetic abnormalities are most likely to occur during cell division. Microtubules, comprised of the cytoskeletal protein tubulin, organize and separate chromosomes during cell division, thus it has been our main imaging target. We have been able to detect chromosomal abnormalities in human embryonic stem cells by fluorescence microscopy.

AB - Ensuring genetic stability in pluripotent stem cell (PSC) cultures is essential for the development of successful cell therapies. Many laboratories have found the emergence of genetic abnormalities in PSCs when cultured in vitro for prolonged amounts of time. These cells are mostly cultured in non-physiological stiff substrates like tissue culture polystyrene which produces the suspicion that the cause of these abnormalities may be influenced by substrate mechanics. In order to verify this, it is important to be able to determine and image, using fluorescence microscopy, potential targets within the cells that are indicative of genetic abnormalities. These genetic abnormalities are most likely to occur during cell division. Microtubules, comprised of the cytoskeletal protein tubulin, organize and separate chromosomes during cell division, thus it has been our main imaging target. We have been able to detect chromosomal abnormalities in human embryonic stem cells by fluorescence microscopy.

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KW - Substrate stiffness

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Imaging targets to identify chromosomal abnormalities in cells

Abstract

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Keywords

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