IL-10 down-regulates human microglial IL-8 by inhibition of NF-κB activation

Laura C. Ehrlich, Shuxian Hu, Phillip K. Peterson, Chun C. Chao

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

IN the present study, we tested the hypothesis that interleukin (IL)-10 down-regulates human microglial cell IL-8 release by inhibiting activation of nuclear factor kappa B (NF-κB). Immunohistochemical staining demonstrated that IL-10 markedly suppressed lipopolysaccharide (LPS)- and IL-1β- stimulated IL-8 expression. NF-≃B involvement was suggested by the finding that pyrrolidinedithiocarbamate, a known inhibitor of NF-κB activation, blocked LPS- and IL-1β-induced IL-8 production. Consistent with our hypothesis, IL-10 treatment of LPS- and IL-1β-stimulated microglia was associated with a marked decrease in NF-κB translocation from the cytoplasm to the nucleus.

Original languageEnglish (US)
Pages (from-to)1723-1726
Number of pages4
JournalNeuroreport
Volume9
Issue number8
DOIs
StatePublished - Jun 1 1998

Keywords

  • IL-10
  • IL-8
  • Inflammation
  • Microglia
  • Transcription factors

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