IGF-I regulates redox status in breast cancer cells by activating the amino acid transport molecule xC-

Yuzhe Yang, Douglas Yee

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Insulin-like growth factors (IGF) stimulate cell growth in part by increasing amino acid uptake. xCT (SLC7A11) encodes the functional subunit of the cell surface transport system xC-, which mediates cystine uptake, a pivotal step in glutathione synthesis and cellular redox control. In this study, we show that IGF-I regulates cystine uptake and cellular redox status by activating the expression and function of xCT in estrogen receptor-positive (ER+) breast cancer cells by a mechanism that relies on the IGF receptor substrate-1 (IRS-1). Breast cancer cell proliferation mediated by IGF-I was suppressed by attenuating xCT expression or blocking xCT activity with the pharmacologic inhibitor sulfasalazine (SASP). Notably, SASP sensitized breast cancer cells to inhibitors of the type I IGF receptor (IGF-IR) in a manner reversed by the reactive oxygen species (ROS) scavenger N-Acetyl-L-cysteine. Thus, IGF-I promoted the proliferation of ER+ breast cancer cells by regulating xC- transporter function to protect cancer cells from ROS in an IRS-1-dependent manner. Our findings suggest that inhibiting xC - transporter function may synergize with modalities that target the IGF-IR to heighten their therapeutic effects.

Original languageEnglish (US)
Pages (from-to)2295-2305
Number of pages11
JournalCancer Research
Volume74
Issue number8
DOIs
StatePublished - Apr 15 2014

Fingerprint Dive into the research topics of 'IGF-I regulates redox status in breast cancer cells by activating the amino acid transport molecule xC<sup>-</sup>'. Together they form a unique fingerprint.

Cite this