Identifying Effective Enzyme Activity Targets for Recombinant Class I and Class II Collagenase for Successful Human Islet Isolation

Appakalai N. Balamurugan, Michael L. Green, Andrew G. Breite, Gopalakrishnan Loganathan, Josh Wilhelm, Benjamin Tweed, Lenka Vargova, Amber D Lockridge, Manikya Kuriti, Michael G. Hughes, Stuart K. Williams, Bernhard J. Hering, Francis E. Dwulet, Robert C. McCarthy

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Background. Isolation following a good manufacturing practice-compliant, human islet product requires development of a robust islet isolation procedure where effective limits of key reagents are known. The enzymes used for islet isolation are critical but little is known about the doses of class I and class II collagenase required for successful islet isolation. Methods. We used a factorial approach to evaluate the effect of high and low target activities of recombinant class I (rC1) and class II (rC2) collagenase on human islet yield. Consequently, 4 different enzyme formulations with divergent C1:C2 collagenase mass ratios were assessed, each supplemented with the same dose of neutral protease. Both split pancreas and whole pancreas models were used to test enzyme targets (n = 20). Islet yield/g pancreas was compared with historical enzymes (n = 42). Results. Varying the Wunsch (rC2) and collagen degradation activity (CDA, rC1) target dose, and consequently the C1:C2 mass ratio, had no significant effect on tissue digestion. Digestions using higher doses of Wunsch and CDA resulted in comparable islet yields to those obtained with 60% and 50% of those activities, respectively. Factorial analysis revealed no significant main effect of Wunsch activity or CDA for any parameter measured. Aggregate results from 4 different collagenase formulations gave 44% higher islet yield (>5000 islet equivalents/g) in the body/tail of the pancreas (n = 12) when compared with those from the same segment using a standard natural collagenase/protease mixture (n = 6). Additionally, islet yields greater than 5000 islet equivalents/g pancreas were also obtained in whole human pancreas. Conclusions. A broader C1:C2 ratio can be used for human islet isolation than has been used in the past. Recombinant collagenase is an effective replacement for the natural enzyme and we have determined that high islet yield can be obtained even with low doses of rC1:rC2, which is beneficial for the survival of islets.

Original languageEnglish (US)
Pages (from-to)E54
JournalTransplantation Direct
Volume2
Issue number1
DOIs
StatePublished - Jan 1 2016

Bibliographical note

Funding Information:
This project was supported by grant number R44DK065467 from the NIDDK. The content is solely the responsibility of the authors and does not necessarily represent the official views of NIDDK or the NIH.

Funding Information:
The authors thank Muhamad Abdullah, Kate Mueller, Tom Gilmore, and William Tucker for technical assistance. Jewish Heritage Fund for Excellence partially supported the human islet isolations performed at the University of Louisville. The authors thank Kentucky Organ Donor Affiliates (KODA) for the supply of human pancreases. Special thanks to Leigh Kleinert, B. Vishaal, Praneeth Goli, Saipruthvi Vanaprthy, Agharnan Gandhi, and Dr. V. Subhashree (VIT University, Vellore) for providing valuable comments.

Funding Information:
This project was supported by grant number R44DK065467 from the NIDDK. The content is solely the responsibility of the authors and does not necessarily represent the official views of NIDDK or the NIH. The authors thank Muhamad Abdullah, Kate Mueller, Tom Gilmore, and William Tucker for technical assistance. Jewish Heritage Fund for Excellence partially supported the human islet isolations performed at the University of Louisville. The authors thank Kentucky Organ Donor Affiliates (KODA) for the supply of human pancreases. Special thanks to Leigh Kleinert, B. Vishaal, Praneeth Goli, Saipruthvi Vanaprthy, Agharnan Gandhi, and Dr. V. Subhashree (VIT University, Vellore) for providing valuable comments.

Publisher Copyright:
© 2015 The Authors.

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