Identification of the cyclin D1b mRNA variant in mouse

Jack Wu; Si Hung Wu; Aliccia Bollig; Archana Thakur; Dezhong J Liao

doi:10.1007/s11033-008-9267-7

Identification of the cyclin D1b mRNA variant in mouse

Jack Wu, Si Hung Wu, Aliccia Bollig, Archana Thakur, Dezhong J Liao

The Hormel Institute

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Cyclin D1 plays a key regulatory role during the G1 phase of the cell cycle and its gene is amplified and over-expressed in many cancers. The cyclin D1b mRNA variant was established in human cells and recent functional analyses revealed that its protein product harbors unique activities in human cancer cells. By performing reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) experiments, we identified the cyclin D1b mRNA variant in mouse. Similar to its human counterpart, the mouse cyclin D1b transcript consists of exon 1, 2, 3, 4 and part of intron 4, and contains a long open reading frame (ORF). The predicted peptide from this ORF is 34-amino acid longer than the human cyclin D1b. The expression of this mouse mRNA variant was investigated. It appears to be expressed ubiquitously and differentially in various mouse cell lines and tissues and its level might be proportional to that of the canonical endogenous cyclin D1a mRNA.

Original language	English (US)
Pages (from-to)	953-957
Number of pages	5
Journal	Molecular Biology Reports
Volume	36
Issue number	5
DOIs	https://doi.org/10.1007/s11033-008-9267-7
State	Published - May 2009

Bibliographical note

Funding Information:
Acknowledgement This work was supported by NIH grant R01CA100864 (J.D.Liao).

Keywords

Cell cycle
DNA sequencing
Exon
Intron
RACE
RT-PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Identification of the cyclin D1b mRNA variant in mouse",

abstract = "Cyclin D1 plays a key regulatory role during the G1 phase of the cell cycle and its gene is amplified and over-expressed in many cancers. The cyclin D1b mRNA variant was established in human cells and recent functional analyses revealed that its protein product harbors unique activities in human cancer cells. By performing reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) experiments, we identified the cyclin D1b mRNA variant in mouse. Similar to its human counterpart, the mouse cyclin D1b transcript consists of exon 1, 2, 3, 4 and part of intron 4, and contains a long open reading frame (ORF). The predicted peptide from this ORF is 34-amino acid longer than the human cyclin D1b. The expression of this mouse mRNA variant was investigated. It appears to be expressed ubiquitously and differentially in various mouse cell lines and tissues and its level might be proportional to that of the canonical endogenous cyclin D1a mRNA.",

keywords = "Cell cycle, DNA sequencing, Exon, Intron, RACE, RT-PCR",

author = "Jack Wu and Wu, {Si Hung} and Aliccia Bollig and Archana Thakur and Liao, {Dezhong J}",

note = "Funding Information: Acknowledgement This work was supported by NIH grant R01CA100864 (J.D.Liao).",

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AU - Wu, Jack

AU - Wu, Si Hung

AU - Bollig, Aliccia

AU - Thakur, Archana

AU - Liao, Dezhong J

N1 - Funding Information: Acknowledgement This work was supported by NIH grant R01CA100864 (J.D.Liao).

PY - 2009/5

Y1 - 2009/5

N2 - Cyclin D1 plays a key regulatory role during the G1 phase of the cell cycle and its gene is amplified and over-expressed in many cancers. The cyclin D1b mRNA variant was established in human cells and recent functional analyses revealed that its protein product harbors unique activities in human cancer cells. By performing reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) experiments, we identified the cyclin D1b mRNA variant in mouse. Similar to its human counterpart, the mouse cyclin D1b transcript consists of exon 1, 2, 3, 4 and part of intron 4, and contains a long open reading frame (ORF). The predicted peptide from this ORF is 34-amino acid longer than the human cyclin D1b. The expression of this mouse mRNA variant was investigated. It appears to be expressed ubiquitously and differentially in various mouse cell lines and tissues and its level might be proportional to that of the canonical endogenous cyclin D1a mRNA.

AB - Cyclin D1 plays a key regulatory role during the G1 phase of the cell cycle and its gene is amplified and over-expressed in many cancers. The cyclin D1b mRNA variant was established in human cells and recent functional analyses revealed that its protein product harbors unique activities in human cancer cells. By performing reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) experiments, we identified the cyclin D1b mRNA variant in mouse. Similar to its human counterpart, the mouse cyclin D1b transcript consists of exon 1, 2, 3, 4 and part of intron 4, and contains a long open reading frame (ORF). The predicted peptide from this ORF is 34-amino acid longer than the human cyclin D1b. The expression of this mouse mRNA variant was investigated. It appears to be expressed ubiquitously and differentially in various mouse cell lines and tissues and its level might be proportional to that of the canonical endogenous cyclin D1a mRNA.

KW - Cell cycle

KW - DNA sequencing

KW - Exon

KW - Intron

KW - RACE

KW - RT-PCR

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Identification of the cyclin D1b mRNA variant in mouse

Abstract

Bibliographical note

Keywords

UN SDGs

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