Identification of the cyclin D1b mRNA variant in mouse

Jack Wu, Si Hung Wu, Aliccia Bollig, Archana Thakur, Dezhong J Liao

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Cyclin D1 plays a key regulatory role during the G1 phase of the cell cycle and its gene is amplified and over-expressed in many cancers. The cyclin D1b mRNA variant was established in human cells and recent functional analyses revealed that its protein product harbors unique activities in human cancer cells. By performing reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) experiments, we identified the cyclin D1b mRNA variant in mouse. Similar to its human counterpart, the mouse cyclin D1b transcript consists of exon 1, 2, 3, 4 and part of intron 4, and contains a long open reading frame (ORF). The predicted peptide from this ORF is 34-amino acid longer than the human cyclin D1b. The expression of this mouse mRNA variant was investigated. It appears to be expressed ubiquitously and differentially in various mouse cell lines and tissues and its level might be proportional to that of the canonical endogenous cyclin D1a mRNA.

Original languageEnglish (US)
Pages (from-to)953-957
Number of pages5
JournalMolecular Biology Reports
Volume36
Issue number5
DOIs
StatePublished - May 1 2009

Keywords

  • Cell cycle
  • DNA sequencing
  • Exon
  • Intron
  • RACE
  • RT-PCR

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