Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Jilin Cheng; Xin Zhou; Tsui Fen Chou; Brahma Ghosh; Baoling Liu; Carston R. Wagner

doi:10.1016/j.bmcl.2009.09.067

Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Jilin Cheng
, Xin Zhou
, Tsui Fen Chou
, Brahma Ghosh
, Baoling Liu
, Carston R. Wagner

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.

Original language	English (US)
Pages (from-to)	6379-6381
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	19
Issue number	22
DOIs	https://doi.org/10.1016/j.bmcl.2009.09.067
State	Published - 2009

Bibliographical note

Funding Information:
This work was partially supported by Grant CA-61909 and the University of Minnesota Graduate School (C.R.W).

Keywords

AZT
Hint1
Phosphoramidate
Prodrug
Pronucleotide

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10.1016/j.bmcl.2009.09.067

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title = "Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection",

abstract = "A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.",

keywords = "AZT, Hint1, Phosphoramidate, Prodrug, Pronucleotide",

author = "Jilin Cheng and Xin Zhou and Chou, \{Tsui Fen\} and Brahma Ghosh and Baoling Liu and Wagner, \{Carston R.\}",

note = "Funding Information: This work was partially supported by Grant CA-61909 and the University of Minnesota Graduate School (C.R.W).",

year = "2009",

doi = "10.1016/j.bmcl.2009.09.067",

language = "English (US)",

volume = "19",

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AU - Cheng, Jilin

AU - Zhou, Xin

AU - Chou, Tsui Fen

AU - Ghosh, Brahma

AU - Liu, Baoling

AU - Wagner, Carston R.

N1 - Funding Information: This work was partially supported by Grant CA-61909 and the University of Minnesota Graduate School (C.R.W).

PY - 2009

Y1 - 2009

N2 - A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.

AB - A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.

KW - AZT

KW - Hint1

KW - Phosphoramidate

KW - Prodrug

KW - Pronucleotide

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UR - https://www.scopus.com/pages/publications/71049166830#tab=citedBy

U2 - 10.1016/j.bmcl.2009.09.067

DO - 10.1016/j.bmcl.2009.09.067

M3 - Article

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AN - SCOPUS:71049166830

SN - 0960-894X

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SP - 6379

EP - 6381

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 22

ER -

Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Abstract

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Keywords

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