Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Jilin Cheng, Xin Zhou, Tsui Fen Chou, Brahma Ghosh, Baoling Liu, Carston R. Wagner

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.

Original languageEnglish (US)
Pages (from-to)6379-6381
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number22
DOIs
StatePublished - Dec 8 2009

Fingerprint

Bacteriophage T7
Bacteriophages
Display devices
Ribonucleotides
Amino Acids
Prodrugs
Enzymes
Libraries
Complementary DNA
phosphoramidic acid

Keywords

  • AZT
  • Hint1
  • Phosphoramidate
  • Prodrug
  • Pronucleotide

Cite this

Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection. / Cheng, Jilin; Zhou, Xin; Chou, Tsui Fen; Ghosh, Brahma; Liu, Baoling; Wagner, Carston R.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 19, No. 22, 08.12.2009, p. 6379-6381.

Research output: Contribution to journalArticle

Cheng, Jilin ; Zhou, Xin ; Chou, Tsui Fen ; Ghosh, Brahma ; Liu, Baoling ; Wagner, Carston R. / Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection. In: Bioorganic and Medicinal Chemistry Letters. 2009 ; Vol. 19, No. 22. pp. 6379-6381.
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