Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Jilin Cheng; Xin Zhou; Tsui Fen Chou; Brahma Ghosh; Baoling Liu; Carston R. Wagner

doi:10.1016/j.bmcl.2009.09.067

Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Jilin Cheng, Xin Zhou, Tsui Fen Chou, Brahma Ghosh, Baoling Liu, Carston R. Wagner

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.

Original language	English (US)
Pages (from-to)	6379-6381
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	19
Issue number	22
DOIs	https://doi.org/10.1016/j.bmcl.2009.09.067
State	Published - 2009

Bibliographical note

Funding Information:
This work was partially supported by Grant CA-61909 and the University of Minnesota Graduate School (C.R.W).

Keywords

AZT
Hint1
Phosphoramidate
Prodrug
Pronucleotide

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10.1016/j.bmcl.2009.09.067

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title = "Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection",

abstract = "A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.",

keywords = "AZT, Hint1, Phosphoramidate, Prodrug, Pronucleotide",

author = "Jilin Cheng and Xin Zhou and Chou, {Tsui Fen} and Brahma Ghosh and Baoling Liu and Wagner, {Carston R.}",

note = "Funding Information: This work was partially supported by Grant CA-61909 and the University of Minnesota Graduate School (C.R.W).",

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doi = "10.1016/j.bmcl.2009.09.067",

language = "English (US)",

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AU - Cheng, Jilin

AU - Zhou, Xin

AU - Chou, Tsui Fen

AU - Ghosh, Brahma

AU - Liu, Baoling

AU - Wagner, Carston R.

N1 - Funding Information: This work was partially supported by Grant CA-61909 and the University of Minnesota Graduate School (C.R.W).

PY - 2009

Y1 - 2009

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AB - A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.

KW - AZT

KW - Hint1

KW - Phosphoramidate

KW - Prodrug

KW - Pronucleotide

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 22

ER -

Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Abstract

Bibliographical note

Keywords

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