Identification of target genes mediated by two-component regulators of staphylococcus aureus using RNA-seq technology

Ting Lei, Junshu Yang, Aaron Becker, Yinduo Ji

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Transcriptomics enables us to elucidate comprehensive gene expression profiles in given experimental conditions. Global regulators, which include transcriptional regulators and two-component regulatory systems, have evolved in a variety of bacterial systems. They play important roles in bacterial fitness and pathogenesis by regulating target gene expression. Advanced next-generation RNA sequencing technology (RNA-seq) provides a powerful and effective tool to analyze the transcriptome of bacterial cells. In this chapter, we provide a detailed procedure for the investigation of gene expression profiles and identification of target genes, regulons, and/or pathways that are mediated by a regulator. This procedure is done using RNA-seq analysis, which involves RNA purification, mRNA enrichment, decontamination, RNA-seq data analysis, and quantitative real-time reverse transcription PCR.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages125-138
Number of pages14
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2069
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

RNA Sequence Analysis
Staphylococcus aureus
Technology
Transcriptome
Genes
Regulon
Decontamination
Gene Expression Profiling
Reverse Transcription
RNA
Gene Expression
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Gene expression profile
  • Real-time reverse transcription polymerase chain reaction
  • RNA sequencing technologies (RNA-seq)
  • Staphylococcus aureus
  • Two-component system (TCS)

PubMed: MeSH publication types

  • Journal Article

Cite this

Lei, T., Yang, J., Becker, A., & Ji, Y. (2020). Identification of target genes mediated by two-component regulators of staphylococcus aureus using RNA-seq technology. In Methods in Molecular Biology (pp. 125-138). (Methods in Molecular Biology; Vol. 2069). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9849-4_10

Identification of target genes mediated by two-component regulators of staphylococcus aureus using RNA-seq technology. / Lei, Ting; Yang, Junshu; Becker, Aaron; Ji, Yinduo.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 125-138 (Methods in Molecular Biology; Vol. 2069).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lei, T, Yang, J, Becker, A & Ji, Y 2020, Identification of target genes mediated by two-component regulators of staphylococcus aureus using RNA-seq technology. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2069, Humana Press Inc., pp. 125-138. https://doi.org/10.1007/978-1-4939-9849-4_10
Lei T, Yang J, Becker A, Ji Y. Identification of target genes mediated by two-component regulators of staphylococcus aureus using RNA-seq technology. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 125-138. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9849-4_10
Lei, Ting ; Yang, Junshu ; Becker, Aaron ; Ji, Yinduo. / Identification of target genes mediated by two-component regulators of staphylococcus aureus using RNA-seq technology. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 125-138 (Methods in Molecular Biology).
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