Identification of some lectin IB4 binding proteins in rat dorsal root ganglia

Joseph M. Fullmer, Maureen S. Riedl, Lee Ann Higgins, Robert Elde

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Lectins are proteins that bind to glycoproteins and glycolipids. The isolectin Griffonia simplicifolia I-B4 (IB4) recognizes terminal α-galactose and binds to a subset of small and medium-sized neurons in the dorsal root ganglia (DRG). Using one and two-dimensional gel electrophoresis, we have identified several proteins that bind IB4 in sciatic nerve, dorsal horn, and DRG. Treatment with the enzyme α-galactosidase reduces IB4 binding, strongly suggesting the binding is specific for the IB4 epitope. Mass spectrometric analysis of tryptic digests of α-galactosidase sensitive bands identified three proteins that bind IB4: the laminin β2 chain and the light and medium subunits of neurofilaments.

Original languageEnglish (US)
Pages (from-to)1705-1709
Number of pages5
JournalNeuroreport
Volume15
Issue number11
DOIs
StatePublished - Aug 6 2004

Keywords

  • Glycoproteins
  • Glycosylation
  • IB4
  • Laminin
  • Neurofilaments
  • Proteomics
  • Rat

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