Identification of sequence alterations in the upstream regulatory region of the estrogen receptor gene in an ER-negative breast cancer cell line

Julia Ann Sullivan; Claudia S. Cohn; Steven M. Hill

doi:10.1016/S0304-3835(97)04606-5

Identification of sequence alterations in the upstream regulatory region of the estrogen receptor gene in an ER-negative breast cancer cell line

Julia Ann Sullivan
, Claudia S. Cohn
, Steven M. Hill

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Given the important role of the estrogen receptor (ER) in the development and physiology of the breast, it is essential to delineate the mechanisms responsible for its failed expression in some breast tumors. We have cloned and sequenced a portion of the ER upstream regulatory region from the ER-positive MCF-7 and the ER-negative MDA-MB-231 breast cancer cell lines to determine if sequence alterations in this region account for the ER-negative phenotype of some tumors. From this, we identified a number of variations between the sequences, two of which were determined to be associated with a 50% decrease in CAT activity.

Original language	English (US)
Pages (from-to)	131-139
Number of pages	9
Journal	Cancer Letters
Volume	113
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(97)04606-5
State	Published - Feb 26 1997

Bibliographical note

Funding Information:
This work was supportedb y a grant BE-72794 from the American Cancer Society to SMH.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Breast cancer
Estrogen receptor
Gene expression

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10.1016/S0304-3835(97)04606-5

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title = "Identification of sequence alterations in the upstream regulatory region of the estrogen receptor gene in an ER-negative breast cancer cell line",

abstract = "Given the important role of the estrogen receptor (ER) in the development and physiology of the breast, it is essential to delineate the mechanisms responsible for its failed expression in some breast tumors. We have cloned and sequenced a portion of the ER upstream regulatory region from the ER-positive MCF-7 and the ER-negative MDA-MB-231 breast cancer cell lines to determine if sequence alterations in this region account for the ER-negative phenotype of some tumors. From this, we identified a number of variations between the sequences, two of which were determined to be associated with a 50\% decrease in CAT activity.",

keywords = "Breast cancer, Estrogen receptor, Gene expression",

author = "Sullivan, \{Julia Ann\} and Cohn, \{Claudia S.\} and Hill, \{Steven M.\}",

note = "Funding Information: This work was supportedb y a grant BE-72794 from the American Cancer Society to SMH.",

year = "1997",

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doi = "10.1016/S0304-3835(97)04606-5",

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pages = "131--139",

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T1 - Identification of sequence alterations in the upstream regulatory region of the estrogen receptor gene in an ER-negative breast cancer cell line

AU - Sullivan, Julia Ann

AU - Cohn, Claudia S.

AU - Hill, Steven M.

N1 - Funding Information: This work was supportedb y a grant BE-72794 from the American Cancer Society to SMH.

PY - 1997/2/26

Y1 - 1997/2/26

N2 - Given the important role of the estrogen receptor (ER) in the development and physiology of the breast, it is essential to delineate the mechanisms responsible for its failed expression in some breast tumors. We have cloned and sequenced a portion of the ER upstream regulatory region from the ER-positive MCF-7 and the ER-negative MDA-MB-231 breast cancer cell lines to determine if sequence alterations in this region account for the ER-negative phenotype of some tumors. From this, we identified a number of variations between the sequences, two of which were determined to be associated with a 50% decrease in CAT activity.

AB - Given the important role of the estrogen receptor (ER) in the development and physiology of the breast, it is essential to delineate the mechanisms responsible for its failed expression in some breast tumors. We have cloned and sequenced a portion of the ER upstream regulatory region from the ER-positive MCF-7 and the ER-negative MDA-MB-231 breast cancer cell lines to determine if sequence alterations in this region account for the ER-negative phenotype of some tumors. From this, we identified a number of variations between the sequences, two of which were determined to be associated with a 50% decrease in CAT activity.

KW - Breast cancer

KW - Estrogen receptor

KW - Gene expression

UR - https://www.scopus.com/pages/publications/0031586750

UR - https://www.scopus.com/pages/publications/0031586750#tab=citedBy

U2 - 10.1016/S0304-3835(97)04606-5

DO - 10.1016/S0304-3835(97)04606-5

M3 - Article

C2 - 9065812

AN - SCOPUS:0031586750

SN - 0304-3835

VL - 113

SP - 131

EP - 139

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

Identification of sequence alterations in the upstream regulatory region of the estrogen receptor gene in an ER-negative breast cancer cell line

Abstract

Bibliographical note

UN SDGs

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