Identification of related peptides recognized by a monoclonal antibody using a synthetic peptide combinatorial library

Jon R. Appel, Clemencia Pinilla, Richard A. Houghten

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Synthetic peptide combinatorial libraries (SPCLs) are composed of tens of millions of peptide sequences from which specific sequences can be systematically determined. The broad versatility of SPCLs residues in the fact that nonsupport-bound peptides are generated in quantities sufficient for use in the majority of existing bioassays. In the present study, an SPCL composed of more than 52 million peptides was screened with an antipeptide monoclonal antibody for the identification of peptide sequences that inhibit the interaction of the monoclonal antibody with its known antigenic peptide. Upon the completion of an iterative screening and selection process utilized in the SPCL approach, a number of peptide sequences that were related to the known antigenic determinant region were found.

Original languageEnglish (US)
Pages (from-to)17-23
Number of pages7
JournalImmunoMethods
Volume1
Issue number1
DOIs
StatePublished - Aug 1992
Externally publishedYes

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