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Identification of proteins differentially expressed in gastric cancer cells with high metastatic potential for invasion to lymph nodes

  • Do Hee Lee
  • , Youra Lee
  • , Joohyun Ryu
  • , Sung Goo Park
  • , Sayeon Cho
  • , Je Jung Lee
  • , Chan Choi
  • , Byoung Chul Park

Research output: Contribution to journalArticlepeer-review

Abstract

In a search for proteins involved in cancer metastasis, we analyzed proteomes of the human gastric cancer cell OCUM-2M and its metastatic subline OCUM-2MLN. We observed that aspartate aminotransferase (AAT), D-site binding protein (DBP), and anterior gradient protein 2 (AGR2) are differentially expressed in metastatic OCUM-2MLN cells. Measurement of protein expression in clinical samples indicated that DBP and AAT are also down-regulated in metastatic adenocarcinoma. Additionally, urokinase-type tissue plasminogen activator is up-regulated in OCUM-2MLN cells and also in metastatic gastric cancer samples. Collectively, these results raise a possibility that AAT, DBP and AGR2 are functionally implicated in the invasiveness of gastric cancer cells.

Original languageEnglish (US)
Pages (from-to)563-571
Number of pages9
JournalMolecules and cells
Volume31
Issue number6
DOIs
StatePublished - Jun 2011
Externally publishedYes

Bibliographical note

Funding Information:
We thank Prof. K. Hirakawa (First Department of Surgery, Osaka City University Medical School) for providing OCUM-2M and OCUM-2MLN cell lines. This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea (BGC0300912) to Byoung Chul Park and a special research grant from Seoul Women’s Univ ersity (2011) to Do Hee Lee.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • AAT
  • AGR2
  • DBP
  • gastric cancer
  • metastasis
  • proteome

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