Abstract
In a search for proteins involved in cancer metastasis, we analyzed proteomes of the human gastric cancer cell OCUM-2M and its metastatic subline OCUM-2MLN. We observed that aspartate aminotransferase (AAT), D-site binding protein (DBP), and anterior gradient protein 2 (AGR2) are differentially expressed in metastatic OCUM-2MLN cells. Measurement of protein expression in clinical samples indicated that DBP and AAT are also down-regulated in metastatic adenocarcinoma. Additionally, urokinase-type tissue plasminogen activator is up-regulated in OCUM-2MLN cells and also in metastatic gastric cancer samples. Collectively, these results raise a possibility that AAT, DBP and AGR2 are functionally implicated in the invasiveness of gastric cancer cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 563-571 |
| Number of pages | 9 |
| Journal | Molecules and cells |
| Volume | 31 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2011 |
| Externally published | Yes |
Bibliographical note
Funding Information:We thank Prof. K. Hirakawa (First Department of Surgery, Osaka City University Medical School) for providing OCUM-2M and OCUM-2MLN cell lines. This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea (BGC0300912) to Byoung Chul Park and a special research grant from Seoul Women’s Univ ersity (2011) to Do Hee Lee.
Keywords
- AAT
- AGR2
- DBP
- gastric cancer
- metastasis
- proteome
