Identification of peptides from human pathogens able to cross-activate an HIV-1-gag-specific CD4+ T cell clone

Sara Venturini, Gina Allicotti, Yindong Zhao, Richard Simon, Dennis R. Burton, Clemencia Pinilla, Pascal Poignard

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Antigen recognition by T cells is degenerate both at the MHC and the TCR level. In this study, we analyzed the cross-reactivity of a human HIV-1 gag p24-specific CD4+ T cell clone obtained from an HIV-1-seronegative donor using a positional scanning synthetic combinatorial peptide library (PS-SCL)-based biometrical analysis. A number of decapeptides able to activate the HIV-1 gag-specific clone were identified and shown to correspond to sequences found in other human pathogens. Two of these peptides activated the T cell done with the same stimulatory potency as the original HIV-1 gag p24 peptide. These findings show that an HIV-1-specific human T helper clone can react efficiently with peptides from other pathogens and suggest that cellular immune responses identified as being specific for one human pathogen (HIV-1) could arise from exposure to other pathogens.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalEuropean Journal of Immunology
Volume36
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

Keywords

  • Combinatorial libraries
  • Cross-reactivity
  • Epitope specificity
  • HIV
  • T cells

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