Identification of oral mitis group streptococci by arbitrarily primed polymerase chain reaction

Joel D Rudney, C. J. Larson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

'Mitis group' streptococci are commensal but may play some role in dental caries, septicemia or endocarditis. Rapid genotypic identification would aid studies of dental plaque ecology, or diagnostic use. AP-PCR with 58 unpaired arbitrary primers was used to characterize 7 Streptococcus gordonii, 11 Streptococcus sanguis, 2 Streptococcus crista, 5 Streptococcus parasanguis, 18 Streptococcus oralis, and 36 Streptococcus mitis (22 biovar 1 and 14 biovar 2). S. parasanguis 16S rRNA variable region primer RR2 produced species-specific bands with all S. gordonii and S. sanguis. Human Vβ1 T-cell receptor primer 434 yielded concordant genotypic identification of all phenotypically defined S. crista and S. parasanguis, 83% of S. oralis, and 74% of S. mitis biovar 1. Amplicon patterns for S. mitis biovar 2 were heterogeneous. Findings suggest that primers RR2 and 434 in succession will allow rapid identification of genotypic groups corresponding closely to mitis group species established by phenotype.

Original languageEnglish (US)
Pages (from-to)33-42
Number of pages10
JournalOral Microbiology and Immunology
Volume14
Issue number1
DOIs
StatePublished - Feb 1 1999

Fingerprint

Streptococcus mitis
Streptococcus oralis
Streptococcus gordonii
Streptococcus sanguis
Polymerase Chain Reaction
Streptococcus
Dental Plaque
Social Identification
Dental Caries
T-Cell Antigen Receptor
Endocarditis
Ecology
Sepsis
Phenotype

Keywords

  • Arbitrarily primed polymerase chain reaction
  • Genotyping
  • Oral streptococci
  • Streptococcus mitis

Cite this

Identification of oral mitis group streptococci by arbitrarily primed polymerase chain reaction. / Rudney, Joel D; Larson, C. J.

In: Oral Microbiology and Immunology, Vol. 14, No. 1, 01.02.1999, p. 33-42.

Research output: Contribution to journalArticle

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