Identification of metabolites diagnostic for organic acidurias by simultaneous dual-column capillary gas chromatography

Michael Y Tsai, Charissa Oliphant, Mark W. Josephson

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

To offer a procedure with increased resolution compared to packed-column gas chromatography, we developed a dual-capillary method of gas chromatography for diagnosing organic acidurias. We derivatized and injected organic acids repeatedly on two different bonded phase columns (DB-1, DB-1701) to establish a table of methylene units. Compounds in urine specimens were identified by their characteristic pair of methylene units. With this method, we are able to identify 120 metabolites in urine. Accordingly, the procedure provides a cost-effective alternative to routine gas chromatography-mass spectrometry.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume341
Issue numberC
DOIs
StatePublished - 1985

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