In an effort to better understand the transcriptional program regulated by transcription factor, AP-2delta, we used cDNA microarray to evaluate the relative expression of human genes in AD293 cells by exogenous expression of AP-2delta. Microarry showed 29 genes that were up-regulated and 39 genes with a down-regulated expression pattern. Among the identified genes were those encoding transcription factors, signal transduction molecules, kinases, as well as genes regulating cell growth, differentiation, and cell adhesion, a set of genes suggested to be regulated by AP-2. The results of microarray was valided for some regulated genes by real-time PCR analysis.These observations shed novel insight into the mechanism of AP-2delta action and provide a range of target genes for further investigation.
|Original language||English (US)|
|Number of pages||8|
|Journal||Frontiers in Bioscience|
|State||Published - 2007|