Identification of francisella noatunensis in novel host species French grunt (Haemulon flavolineatum) and caesar grunt (Haemulon carbonarium)

Esteban Soto, Alexander E. Primus, Deborah B. Pouder, Robert H. George, Trevor J. Gerlach, Stephen E. Cassle, Tammy Johnson, Sean Boyd, Tim Handsel, Roy P E Yanong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Francisella noatunensis is an emerging pathogen of fish that has been isolated from several cultured species worldwide. Here presented is a case involving several hundred marine grunts that were caught near the Florida Keys for display in public aquaria. These fish were maintained in a recirculating system where they began to experience mortalities approximately two weeks post-stocking. Postmortem examination revealed disseminated systemic granulomatous disease most severely affecting spleen and kidney. Splenic and renal tissue homogenates inoculated in modified Thayer Martin agar media yielded colonies consistent with F. noatunensis 4 days post inoculation. Bacterial colonies and tissues were confirmed positive after real-time PCR amplification of the intracellular growth loci gene (iglC) specific for F. noatunensis subspecies orientalis. Consequently, multiple novel host species for this pathogen were identified, including the French grunt (Haemulon flavolineatum) and the Caesar grunt (Haemulon carbonarium).

Original languageEnglish (US)
Pages (from-to)727-731
Number of pages5
JournalJournal of Zoo and Wildlife Medicine
Volume45
Issue number3
DOIs
StatePublished - Jan 1 2014

Fingerprint

Francisella
Haemulon
Haemulidae
Fishes
Kidney
kidneys
Agar
Real-Time Polymerase Chain Reaction
Autopsy
recirculating aquaculture systems
Spleen
pathogens
fish
aquariums
Mortality
necropsy
spleen
quantitative polymerase chain reaction
Growth
agar

Keywords

  • Florida
  • Francisella
  • Grunt
  • Temperature

Cite this

Identification of francisella noatunensis in novel host species French grunt (Haemulon flavolineatum) and caesar grunt (Haemulon carbonarium). / Soto, Esteban; Primus, Alexander E.; Pouder, Deborah B.; George, Robert H.; Gerlach, Trevor J.; Cassle, Stephen E.; Johnson, Tammy; Boyd, Sean; Handsel, Tim; Yanong, Roy P E.

In: Journal of Zoo and Wildlife Medicine, Vol. 45, No. 3, 01.01.2014, p. 727-731.

Research output: Contribution to journalArticle

Soto, Esteban ; Primus, Alexander E. ; Pouder, Deborah B. ; George, Robert H. ; Gerlach, Trevor J. ; Cassle, Stephen E. ; Johnson, Tammy ; Boyd, Sean ; Handsel, Tim ; Yanong, Roy P E. / Identification of francisella noatunensis in novel host species French grunt (Haemulon flavolineatum) and caesar grunt (Haemulon carbonarium). In: Journal of Zoo and Wildlife Medicine. 2014 ; Vol. 45, No. 3. pp. 727-731.
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